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目的:探讨王不留行总黄酮对离体蛙心的影响及可能的作用机制。方法:采用斯氏蛙心灌流法制备离体蛙心标本,待离体蛙心收缩力稳定后,分别加入不同浓度的王不留行总黄酮、阿托品、盐酸异丙肾上腺素,观察这些药物对离体蛙心收缩活动的影响。结果:总黄酮在0.02~0.10 mg·mL~(-1)范围内,随着王不留行总黄酮浓度的增大,离体蛙心心肌平均收缩力与给药前相比较明显减小(P<0.05或P<0.01);心率与给药前相比较,仅0.08 mg·mL~(-1)和0.10 mg·mL~(-1)剂量组与给药前比较减慢差异明显(P<0.05或P<0.01);心肌最大收缩力与给药前相比较,除0.04 mg·mL~(-1)剂量组外,其他4组与给药前比较均有明显抑制作用(P<0.01)。随着细胞外钙离子浓度的逐渐增大,王不留行总黄酮对蛙心心肌平均收缩力的抑制率逐渐增大(P<0.05或P<0.01),加入阿托品和盐酸异丙肾上腺素后提取液对离体蛙心的抑制作用有所降低。结论:王不留行总黄酮对离体蛙心活动有一定的抑制作用,作用机制可能与钙离子通道有关,与心肌细胞膜上受体关系有待进一步研究。
Objective: To investigate the effects of flavonoids from Wangbuyu line on isolated frog hearts and its possible mechanism. Methods: The isolated frog heart specimens were prepared by the method of Frog heart perfusion. After the contractile force of the isolated frog heart was stabilized, different concentrations of total flavonoids, atropine and isoproterenol were added into the frog hearts to observe the effects of these drugs Effects of isolated frog heart contraction. Results: The total flavonoids in the range of 0.02 ~ 0.10 mg · mL ~ (-1), with the increase of the total flavonoids concentration of Wangbuyu, the average contractile force of the isolated frog heart muscle was significantly reduced compared with that before administration P <0.05 or P <0.01). Compared with the pre-administration, only 0.08 mg · mL -1 and 0.10 mg · mL -1 significantly decreased heart rate (P <0.05 or P <0.01). Compared with the pre-administration, the maximal contractile force of myocardium was significantly inhibited except 0.04 mg · mL -1 (P <0.01) ). With the increase of extracellular calcium concentration, the inhibitory rate of total flavonoids of Cardiffia ulmoides increased gradually (P <0.05 or P <0.01). After adding atropine and isoprenaline hydrochloride Extract on isolated frog heart inhibition decreased. CONCLUSION: The total flavonoids from Wangbuyu line can inhibit the activity of isolated frog heart in a certain extent. The mechanism may be related to the calcium channels and the relationship with the receptor on the myocardial cell membrane needs to be further studied.