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本文对人腭扁桃体应用Muller低温恒冷切片和邢俭级石蜡切片“ANAE”法标记淋巴细胞。结果:(1)两种方法一致显示淋巴小结生发中心以B淋巴细胞为主,胸腺依赖区以T淋巴细胞为主。(2)胸腺依赖区T淋巴细胞为粗大圆点状颗粒,淋巴小结T淋巴细胞为分散细小颗粒。本法为淋巴器官内T、B淋巴细胞的定性、定位、定量及淋巴器官免疫应答性质和功能状态的组织学研究提供可靠手段。
In this paper, mutilatory tonsils were fixed with Muller cryostat and lymph node sections labeled “ANAE”. Results: (1) Both methods showed that B lymphocyte was the main center of lymphoid germinal center and T lymphocyte was the thymus dependent area. (2) thymus-dependent T lymphocytes are coarse dot-like particles, lymph node T lymphocytes are scattered fine particles. This method provides a reliable means for the qualitative, localization, quantification of T and B lymphocytes in lymphoid organs and the histological study on the functional status and immune response of lymphoid organs.