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目的探讨双歧杆菌脂磷壁酸(LTA)对黑色素瘤B16荷瘤小鼠NK细胞受体NKG2D及其配体的影响。方法将黑色素瘤B16细胞接种于C57BL/6小鼠皮下,待触及肿块后于荷瘤小鼠皮下注射双歧杆菌LTA。采用MTT、流式细胞术(FCM)、RT-PCR方法分别检测经双歧杆菌LTA处理后B16荷瘤小鼠NK细胞杀伤活性、NK细胞NKG2D受体蛋白表达以及肿瘤组织内Rae-1、H60 mRNA表达的变化。结果与对照组相比,经双歧杆菌LTA处理后,B16荷瘤小鼠的NK细胞杀伤活性增强(P<0.05),NK细胞受体NKG2D表达明显增加(P<0.05),肿瘤组织Rae-1、H60 mRNA表达上升(P<0.05),并具有浓度依赖性。结论双歧杆菌LTA能够增强B16荷瘤小鼠NK细胞的杀伤活性,其机制可能与上调NK细胞受体NKG2D的蛋白表达和肿瘤组织Rae-1、H60 mRNA的表达有关。
Objective To investigate the effect of lipoteichoic acid (LTA) on NKG2D receptor and its ligand in B16 tumor-bearing mice of melanoma. Methods Melanoma B16 cells were inoculated subcutaneously in C57BL / 6 mice, and BTA was injected subcutaneously into the tumor-bearing mice until the lumps were touched. The killing activity of NK cells, the expression of NKG2D receptor protein in NK cells and the expressions of Rae-1 and H60 in tumor tissues of B16 tumor-bearing mice were detected by MTT, FCM and RT-PCR respectively. Changes in mRNA expression. Results Compared with the control group, the NK cell killing activity of B16-bearing mice increased (P <0.05) and the expression of NKG2D receptor of NK cells increased significantly (P <0.05) after treatment with Bifidobacterium LTA. The Rae- 1, H60 mRNA expression increased (P <0.05), and in a concentration-dependent manner. Conclusion Bifidobacterium LTA can enhance the killing activity of NK cells in B16 tumor-bearing mice. The mechanism may be related to the up-regulation of NKG2D protein expression and the expression of Rae-1 and H60 mRNA in tumor tissues.