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目的:研究Apogossypolone(ApoG2)对人胃癌细胞系SGC7901体外增殖和凋亡的作用。方法:采用MTT法检测浓度分别为3.125、6.250、12.500、25.000和50.000μmol/L的ApoG2作用于SGC7901细胞24、48和72h后对细胞生长的影响;MGG染色法观察10、20和30μmol/L ApoG2作用24h后细胞形态学的变化;流式细胞术检测10、20、30和40μmol/L ApoG2作用24h后细胞凋亡;RT-PCR方法检测10、20和30μmol/L ApoG2作用细胞24h后,SGC7901细胞中Bcl-2、Bax和NF-κB mRNA表达量的变化。结果:随着药物浓度的增大,ApoG2抑制胃癌细胞增殖的作用逐渐增强,呈剂量依赖性(P<0.05),24、48和72h的半数抑制浓度分别为32.58、25.11和14.16μmol/L;MGG染色可见,随着药物浓度的增大,细胞的生长变慢,胞质变疏松,细胞核深染,核质比例增大,出现典型的细胞死亡形态。流式细胞术检测细胞凋亡可见,经不同浓度的ApoG2处理24h后,SGC7901细胞发生凋亡,0μmol/L早期凋亡率为(1.92±0.55)%,晚期凋亡率为(2.80±0.86)%,10μmol/L早期凋亡率为(3.36±0.55)%,晚期凋亡率为(13.09±0.93)%,20μmol/L早期凋亡率为(5.07±0.70)%,晚期凋亡率为(16.48±1.03)%,30μmol/L早期凋亡率为(7.44±1.47)%,晚期凋亡率为(18.32±1.44)%,40μmol/L早期凋亡率为(7.88±1.22)%,晚期凋亡率为(25.49±1.59)%,随药物浓度的增加凋亡率明显增高,P值均<0.01;RT-PCR检测结果显示,药物干预后,胃癌细胞中Bcl-2和NF-κB的mRNA表达降低,而Bax的表达量升高,差异有统计学意义,P<0.05。结论:ApoG2在体外具有抑制胃癌细胞SGC7901的增殖并杀伤肿瘤细胞的作用。
AIM: To investigate the effects of Apogossypolone (ApoG2) on proliferation and apoptosis of human gastric cancer cell line SGC7901 in vitro. Methods: The effects of ApoG2 at concentrations of 3.125,6.250,12.500,25.000 and 50.000μmol / L on the growth of SGC7901 cells were observed by MTT assay at 24, 48 and 72 hours respectively. MGG staining was used to observe the effects of 10,20 and 30μmol / L Apoptosis of ApoG2 cells was detected by flow cytometry (FCM). The apoptosis of ApoG2 cells treated with 10, 20, 30 and 40 μmol / L ApoG2 was detected by flow cytometry. ApoG2-treated cells were treated with 10, 20 and 30 μmol / Changes of Bcl-2, Bax and NF-κB mRNA expression in SGC7901 cells. Results: With the increase of drug concentration, ApoG2 could inhibit the proliferation of gastric cancer cells in a dose - dependent manner (P <0.05). The half inhibitory concentrations of ApoG2 were 32.58, 25.11 and 14.16 μmol / L at 24, 48 and 72 h, MGG staining shows that, with the increase of drug concentration, the cell growth becomes slow, the cytoplasm becomes loose, the nucleus stained, the proportion of nuclear mass increases, the typical cell death morphology. Flow cytometry showed that apoptosis of SGC7901 cells was induced by ApoG2 at various concentrations for 24 h. The apoptotic rates of early apoptotic cells (0μmol / L) were (1.92 ± 0.55)% and that of late apoptotic cells was (2.80 ± 0.86) % And 10μmol / L respectively, the rate of early apoptosis was (3.36 ± 0.55)%, the rate of late apoptosis was (13.09 ± 0.93)% and the rate of early apoptosis was (5.07 ± 0.70)% 16.48 ± 1.03)%. The early apoptotic rate was (7.44 ± 1.47)% at 30μmol / L, the late apoptosis rate was (18.32 ± 1.44)%, the early apoptosis rate at 40μmol / L was (7.88 ± 1.22) The apoptosis rate was (25.49 ± 1.59)%, with the increase of drug concentration, the apoptosis rate was significantly increased (P <0.01). The results of RT-PCR showed that the mRNA expression of Bcl-2 and NF- While the expression of Bax increased, the difference was statistically significant, P <0.05. Conclusion: ApoG2 can inhibit the proliferation of gastric cancer cell SGC7901 and kill tumor cells in vitro.