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研究了从独子藤中提取雷公藤红素的关键提取参数,包括提取时间(10–20 min),提取温度(30–60°C),超声功率(60–90 W)和液料比(10–30 m L/g)对提取效果的影响。采用Box-Behnken Design设计实验方案,通过响应面法,进一步考察了溶剂种类对提取效果的影响。结果表明,在所选的溶剂甲醇、乙醇、乙酸乙酯、正丁醇和水中,乙醇为最佳提取溶剂。采用二阶多项式模型进行数据分析,模型的相关系数R2=0.9928。确定了独子藤中雷公藤红素的较优提取工艺条件为:提取时间20 min,提取温度46°C,提取功率60 W,液料比30 m L/g,按此条件雷公藤红素的提取率可达3.116 mg/g。预测值和实验实测值之间相关性很强。结果表明,应用响应面法优化超声辅助提取独子藤中雷公藤红素的方法高效且可靠。
The key extraction parameters of tripterine extracted from single cane were studied, including extraction time (10-20 min), extraction temperature (30-60 ° C), ultrasonic power (60-90 W) and liquid to material ratio (10 -30 m L / g) on the extraction effect. The Box-Behnken Design was used to design the experimental scheme, and the effect of solvent type on the extraction effect was further investigated by response surface methodology. The results showed that ethanol was the best extraction solvent in the selected solvents methanol, ethanol, ethyl acetate, n-butanol and water. The second-order polynomial model was used for data analysis. The correlation coefficient of the model was R2 = 0.9928. The optimum extraction conditions of tripterine were found to be as follows: extraction time 20 min, extraction temperature 46 ° C, extraction power 60 W, liquid ratio 30 m L / g, according to this condition, Extraction rate of up to 3.116 mg / g. The correlation between predicted and experimental values is very strong. The results showed that the method of response surface methodology (RSM) to optimize the sonication-assisted extraction of tripterine was feasible and reliable.