Haynaldia villosa possesses a lot of important agronomic traits and has been a powerful gene resource for wheatimprovement.However,only several wheat-H,villosa translocation lines have been reported so far.In this study,weattempted to develop an efficient method for inducing wheat-H,villosa chromosomal translocations.Triticum durum-Haynaldia villosa amphiploid pollen treated with 1 200 rad ~(60)Co-γ-rays was pollinated to Triticum aestivum cv.‘ChineseSpring’.Ninety-eight intergeneric translocated chromosomes between T.durum and H.villosa were detected by genomicin situ hybridization in 44 of 61 M_1 plants,indicating a translocation occurrence frequency of 72.1%;much higher than everreported.There were 26,62 and 10 translocated chromosomes involving whole arm transiocations,terminal translocations,and intercarlary translocations,respectively.Of the total 108 breakage-fusion events,79 involved interstitial regions and29 involved centric regions.The ratio of small segment terminal translocations(W.W-V)was much higher than that oflarge segment terminal translocations(W-V.V).All of the M_1 plants were self-sterile,and their backcross progeny wasall obtained with‘Chinese Spring’as pollen donors.Transmission analysis showed that most of the translocations weretransmittable.This study provides a new strategy for rapid mass production of wheat-alien chromosomal translocations,especially terminal translocations that will be more significant for wheat improvement. Haynaldia villosa possesses a lot of important agronomic traits and has been a powerful gene resource for wheatimprovement. Although, only several wheat-H, villosa translocation lines have been reported so far. In this study, weattempted to develop an efficient method for inducing wheat- H, villosa chromosomal translocations. Triticum durum-Haynaldia villosa amphiploid pollen treated with 1 200 rad ~ (60) Co-γ-rays was pollinated to Triticum aestivum cv. ’Chinese Spring’ .Ninety-eight intergeneric translocated chromosomes between T.durum and H .villosa were detected by genomicin situ hybridization in 44 of 61 M_1 plants, indicating a translocation occurrence frequency of 72.1%; much higher than everreported. There were 26,62 and 10 translocated chromosomes involving whole arm transiocations, terminal translocations, and intercarlary translocations, respectively. Total 108 breakage-fusion events, 79 involved interstitial regions and 29 involving centric regions. The ratio of small segment terminal tr anslocations (WW-V) was much higher than that of large segment terminal translocations (WV.V). All of the M_1 plants were self-sterile, and their backcross progeny wasall obtained with’Chinese Spring’as pollen donors. Transmission analysis showed that that most of the translocations weretransmittable.This study provides a new strategy for rapid mass production of wheat-alien chromosomal translocations, especially terminal translocations that will be more significant for wheat improvement.