【摘 要】
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A new kind of comblike copolymer film composed of acrylic acid-polyethylene glycol monomethyl ether acrylate copolymer(AA-PEGMA copolymer) was successfully synt
【机 构】
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College of Chemistry
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A new kind of comblike copolymer film composed of acrylic acid-polyethylene glycol monomethyl ether acrylate copolymer(AA-PEGMA copolymer) was successfully synthesized to immobilize hemoglobin(Hb).FTIR,UV-Vis and CD spectra suggest that Hb kept its original structure in the AA-PEGMA copolymer film without denaturation.A pair of well-defined,quasi-reversible cyclic voltammetric peaks at around -270 mV vs.saturated calomel electrode(SCE) for the Hb Fe(Ⅲ)/Fe(Ⅱ) redox couple was observed on the film-modified electrode in phosphate buffer solution(PBS,pH=7.0).The formal potential of Hb/AA-PEGMA copolymer film-modified electrode is linearly dependent on solution pH with a slope of-46.3 mV/pH,illustrating that one-proton transfer was accompanied with each electron transfer.Furthermore,the modified electrode displayed electrocatalytic response to the reduction of H2O2 with a linear range of 3.5-126 μmol/L and a detection limit of 1.17 μmol/L.In conclusion,the AA-PEGMA copolymer film was proved to be an excellent matrix for the immobilization and electrochemistry of proteins.
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