恶性肿瘤作为一种严重威胁人类健康的疾病,虽然癌基因的表达与基因突变对恶性肿瘤的发生、发展及预后等有密切关系,但病因至今仍不完全明确。而某些肿瘤相关基因的表达和(或)突变常常是通过细胞体内信号转导系统和细胞膜离子通道实现的。由此可以设想癌基因、离子通道和肿瘤之间可能存在某种联系。膜片钳技术为了解细胞膜的电生理学和药理学特性提供了最直接的手段。该技术 As a serious disease that threatens human health, malignant tumors have not yet been completely elucidated. Although the expression of oncogenes is closely related to the occurrence, development and prognosis of malignant tumor, The expression and / or mutation of certain tumor-associated genes are often mediated by intracellular signal transduction systems and membrane ion channels. It can be assumed that there may be some connection between oncogenes, ion channels and tumors. Patch clamp technology provides the most direct means of understanding the electrophysiological and pharmacological properties of cell membranes. This technique