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目的:研究癫痫模型大鼠中prepro-orexin及其神经纤维在不同时间点的变化情况,以阐明prepro-orexin在癫痫发生中的作用,深化癫痫的发病机制。方法:本研究采用海人酸腹腔注射诱发大鼠癫痫发作,并分别于癫痫终止后8小时、1、3、7天和慢性复发时间点行免疫组化方法检测prepro-orexin免疫反应阳性细胞数及其神经纤维的变化情况。结果:prepro-orexin免疫阳性细胞的分布主要在外侧下丘脑和穹窿周围核,在海马、大脑皮层及其他大脑组织中并未检测到,各组之间prepro-orexin阳性细胞数进行方差分析表明其差异并不显著,P>0.05;而其免疫阳性神经纤维主要分布在下丘脑、丘脑室旁核及海马,数量稀少;结论:大鼠致痫后,随着时间的推移,prepro-orexin免疫阳性细胞总体减少,但未具有统计学意义,但并不能完全认为其与癫痫发作无关;而其免疫反应神经纤维稀少。
OBJECTIVE: To study the changes of prepro-orexin and its nerve fibers at different time points in epileptic rats to clarify the role of prepro-orexin in epilepsy and to deepen the pathogenesis of epilepsy. Methods: In this study, intraperitoneal injection of kainate acid induced seizures in rats. Immunohistochemistry was used to detect the number of prepro-orexin-positive cells at 8 hours, 1, 3, and 7 days after the termination of epilepsy, respectively. And its changes in nerve fibers. RESULTS: The distribution of prepro-orexin immunoreactive cells was mainly located in the lateral hypothalamus and vault, and was not detected in the hippocampus, cerebral cortex and other brain tissues. The variance analysis of prepro-orexin positive cells among the groups showed that The difference was not significant, P> 0.05; while the immunoreactive nerve fibers were mainly distributed in the hypothalamus, paraventricular nucleus and hippocampus, and the quantity was scarce. Conclusion: After the epilepsy in rats, prepro-orexin positive cells Overall reduction, but not statistically significant, but can not fully believe that it has nothing to do with seizures; and its immune response to nerve fiber sparse.