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目的探讨血清磷脂酰肌醇蛋白聚糖3(Glypican 3,GPC3)联合甲胎蛋白异质体(alpha fetoprotein variants,AFP-L3)占甲胎蛋白(alpha fetoprotein,AFP)的百分比(AFP-L3%)在原发性肝癌诊断中的价值。方法原发性肝癌患者43例为肝癌组,慢性肝炎患者43例为肝炎组,肝硬化患者43例为肝硬化组,体检健康者43例为对照组,分别采用ELISA法和亲和吸附法检测4组GPC3和AFP-L3水平,计算AFP-L3%,并比较分析。结果血清GPC3和AFP-L3%从高到低依次为肝癌组((10.94±0.58)μg/L和(15.85±2.74)%)、肝硬化组((5.56±0.49)μg/L和(10.72±2.65)%)、肝炎组((2.73±0.46)μg/L和(9.57±2.59)%)、对照组((1.15±0.42)μg/L和(9.08±2.55)%),肝炎组与对照组AFP-L3%比较差异无统计学意义(P>0.05),其他各组间上述指标比较差异均有统计学意义(P<0.05);与组织病理结果进行对照,AFP-L3%联合GPC3诊断原发性肝癌的符合率(81.40%)明显高于二者单独检测(48.84%、25.58%)(P<0.01)。结论血清GPC3与AFP-L3%联合检测可提高原发性肝癌早期诊断准确率。
Objective To investigate the percentage of serum AFP-L3 (AFP-L3%) associated with Glypican 3 (GPC3) and alpha fetoprotein variants (AFP- ) In the diagnosis of primary liver cancer value. Methods Forty-three cases of primary liver cancer were liver cancer group, 43 cases of chronic hepatitis were hepatitis group, 43 cases of liver cirrhosis were cirrhosis group, and 43 cases of healthy control group were used as control group. ELISA and ELISA Group GPC3 and AFP-L3 levels, calculated AFP-L3%, and comparative analysis. Results The percentages of serum GPC3 and AFP-L3% in the liver cancer group were (10.94 ± 0.58) μg / L and (15.85 ± 2.74)%, respectively, and those in the cirrhosis group were (5.56 ± 0.49) μg / L and (2.65)%, hepatitis group (2.73 ± 0.46) μg / L and (9.57 ± 2.59)%), control group (1.15 ± 0.42μg / L and (9.08 ± 2.55)%) (P> 0.05). There was significant difference among the other groups (P <0.05). Compared with histopathological results, AFP-L3% combined with GPC3 The coincidence rate of HCC (81.40%) was significantly higher than that of HCC alone (48.84%, 25.58%) (P <0.01). Conclusion Combined detection of serum GPC3 and AFP-L3% can improve the accuracy of early diagnosis of primary liver cancer.