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目的:观察肿瘤坏死因子样弱凋亡诱导因子(TWEAK)对人肝星状细胞(HSCs)株LX-2增殖、细胞周期、细胞凋亡、迁移及粘附能力的影响。方法:用不同浓度TWEAK培养LX-2细胞24或48 h,采用CCK-8法检测TWEAK对LX-2细胞增殖的影响,流式细胞术检测TWEAK对LX-2细胞周期和凋亡的影响,Transwell小室检测TWEAK对LX-2细胞迁移能力的影响,Matrigel基质胶检测TWEAK对LX-2细胞的粘附能力的影响。结果:与对照组相比,40 ng/m L和100 ng/m L TWEAK都能使LX-2细胞的迁移能力增强(P<0.05),且100 ng/m L较40 ng/m L作用更强(P<0.01);100 ng/m L TWEAK能够明显抑制LX-2细胞的粘附能力(P<0.0001);40 ng/m L、100 ng/m L TWEAK对LX-2细胞的增殖、周期及凋亡无明显影响(P>0.05)。结论:TWEAK能够增强LX-2细胞迁移能力,抑制其粘附能力。
Objective: To observe the effect of tumor necrosis factor-like weak inducing factor (TWEAK) on the proliferation, cell cycle, apoptosis, migration and adhesion of human hepatic stellate cell (HSC) strain LX-2. Methods: LX-2 cells were cultured with different concentrations of TWEAK for 24 or 48 hours. The effects of TWEAK on the proliferation of LX-2 cells were detected by CCK-8. The effect of TWEAK on the cell cycle and apoptosis of LX-2 cells was detected by flow cytometry. The effect of TWEAK on the migration ability of LX-2 cells was detected by Transwell chamber. The effect of TWEAK on the adhesion of LX-2 cells was detected by Matrigel matrigel. Results: Compared with the control group, the migration ability of LX-2 cells was enhanced by both 40 ng / mL and 100 ng / mL TWEAK (P <0.05), and 100 ng / mL was more effective than 40 ng / mL (P <0.01). TWEAK of 100 ng / m L could significantly inhibit the adhesion of LX-2 cells (P <0.0001). The proliferation of LX-2 cells induced by 40 ng / mL and 100 ng / , Cycle and apoptosis had no significant effect (P> 0.05). Conclusion: TWEAK can enhance the migration ability of LX-2 cells and inhibit its adhesion ability.