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目的研究胃泌素释放肽受体(GRPR)和核因子-κB(NF-κB)在宫颈癌、宫颈上皮内瘤变(CIN)及宫颈鳞状上皮组织中的表达。方法采用免疫组化SP法检测GRPR和NF-κB在宫颈癌、宫颈上皮内瘤变和正常宫颈鳞状上皮组织中的表达的情况。结果①GRPR和NF-κB在宫颈癌中呈阳性表达(64.81%、74.1%),宫颈上皮内瘤变中呈中等量表达(63.64%、46.9%),GRPR在正常宫颈鳞状上皮组织中呈弱阳性表达(15.0%),NF-κB在正常宫颈鳞状上皮组织中无表达(P<0.05);②GRPR和NF-κB在CINⅠ,CINⅡ,CINⅢ中阳性表达呈上升趋势,差别无统计学意义;③GRPR和NF-κB与宫颈癌的临床分期显著相关,并随肿瘤浸润深度的增加,阳性率增高;GRPR与肿瘤分化程度和淋巴结转移呈显著相关,并且与肿瘤恶性程度呈正相关;④GRPR阳性组中,NF-κB阳性率为85.7%,GRPR阴性组中,NF-κB阳性率为52.6%,二者差别有统计学意义。结论 GRPR和NF-κB过度表达,在宫颈癌的发生、发展过程中具有重要的作用,GRPR和NF-κB表达水平随宫颈癌恶性程度的增加而增加;NF-κB表达增加同时GRPR表达增加,二者具有正相关性,联测可作判定肿瘤恶性程度、预后及靶向治疗的新靶标。
Objective To study the expression of gastrin-releasing peptide receptor (GRPR) and nuclear factor-κB (NF-κB) in cervical carcinoma, cervical intraepithelial neoplasia (CIN) and cervical squamous epithelium. Methods The expression of GRPR and NF-κB in cervical cancer, cervical intraepithelial neoplasia and normal cervical squamous epithelium were detected by immunohistochemical SP method. Results (1) The expressions of GRPR and NF-κB in cervical carcinoma were positive (64.81%, 74.1%), moderately expressed in cervical intraepithelial neoplasia (63.64%, 46.9%), and GRPR was weak in normal cervical squamous epithelium The positive expression of NF-κB in normal cervical squamous epithelium (P <0.05); ②The positive expression of GRPR and NF-κB in CINⅠ, CINⅡ and CINⅢ showed an upward trend, but the difference was not statistically significant; GRPR and NF-κB were significantly correlated with the clinical stage of cervical cancer, and with the depth of tumor invasion, the positive rate was increased; GRPR was significantly correlated with tumor differentiation and lymph node metastasis, and positively correlated with the degree of malignancy; ④GRPR-positive group , The positive rate of NF-κB was 85.7%, the positive rate of NF-κB in GRPR negative group was 52.6%, the difference was statistically significant. Conclusions The overexpression of GRPR and NF-κB plays an important role in the carcinogenesis and progression of cervical cancer. The expression of GRPR and NF-κB increased with the malignant degree of cervical cancer. The expression of GRPR and NF-κB increased, The two have a positive correlation, the joint test can be used to determine the degree of malignancy, prognosis and targeted therapy of new targets.