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目的:制备以Fe/Fe3O4核壳结构纳米颗粒(Fe/Fe3O4 core-shell structural magnetic nanoparticles,FCSN)为磁介质的5-氟尿嘧啶(5-FU)磁性脂质体,并检测其表征。方法:使用Fe3O4纳米颗粒通过还原氧化法制备FCSN,用透射电镜(TEM)、X射线衍射仪(XRD)及振动样品磁强计(VSM)检测其形态、结构和磁性。采用逆向蒸发法制备5-FU-FCSN磁性脂质体,通过L9(34)正交表试验得出5-FU-FCSN磁性脂质体最佳配方。用激光粒度分析仪(PCS)、TEM检测5-FU-FCSN磁性脂质体表征,并用凝胶层析法测定包封率。结果:FCSN粒径平均为70 nm,呈类圆形。XRD和TEM结果显示FCSN结构:外壳为Fe3O4,内核为Fe,磁饱和强度为107.54 emu/g。5-FU-FCSN磁性脂质体在TEM下观察为类圆形,平均粒径为202.5 nm,包封率为33.5%。放置在4℃冰箱中,1个月后包封率较为稳定,瓶底无磁性脂质体沉淀。结论:成功制备了5-FU-FCSN磁性脂质体,制备方法简单易行,制备的磁性脂质体在4℃冰箱中可以长期储存。
OBJECTIVE: To prepare 5-fluorouracil (5-FU) magnetic liposomes with Fe / Fe3O4 core-shell structural magnetic nanoparticles (FCSN) as the magnetic medium and to detect the characterization. Methods: FCSN was prepared by reduction oxidation method using Fe3O4 nanoparticles. The morphology, structure and magnetism of FCSN were detected by transmission electron microscopy (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The 5-FU-FCSN magnetic liposomes were prepared by reverse evaporation. The best formulation of 5-FU-FCSN magnetic liposomes was obtained by L9 (34) orthogonal test. 5-FU-FCSN magnetic liposomes were characterized by laser particle size analyzer (PCS) and TEM. The encapsulation efficiency was determined by gel chromatography. Results: The average diameter of FCSN was 70 nm and was round. The results of XRD and TEM show that the structure of FCSN is: the shell is Fe3O4, the core is Fe, and the magnetic saturation intensity is 107.54 emu / g. The 5-FU-FCSN magnetic liposomes were observed as round-shaped under the TEM, the average particle size was 202.5 nm, and the encapsulation efficiency was 33.5%. Placed in refrigerator at 4 ℃, 1 month after the encapsulation efficiency is more stable, the bottom of the bottle without magnetic liposome precipitation. Conclusion: 5-FU-FCSN magnetic liposomes were successfully prepared. The preparation method is simple and easy. The prepared magnetic liposomes can be stored in refrigerator at 4 ℃ for a long time.