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目的探讨乳腺癌组织及其正常乳腺组织中ING4基因表达及其意义。方法采用逆转录-聚合酶链反应(RT-PCR)检测25例伴淋巴结转移的乳腺癌、40例无淋巴结转移的乳腺癌及20例癌旁正常组织中ING4 mRNA表达。结果乳腺癌组织中ING4 mRNA表达水平显著低于癌旁正常组织(P<0.001)。且随着淋巴结转移的出现,ING4 mRNA表达水平逐渐降低,各组间差异具有显著性意义(P<0.01)。ING4 mRNA表达量随乳腺癌的临床分期增高而降低。结论ING4基因可作为乳腺癌的重要生物学指标,有助于对乳腺癌生物学行为的了解,从而指导临床治疗。
Objective To investigate the expression of ING4 gene in breast cancer tissues and normal breast tissues and its significance. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of ING4 mRNA in 25 cases of breast cancer with lymph node metastasis, 40 cases of breast cancer without lymph node metastasis and 20 cases of adjacent normal tissues. Results The expression level of ING4 mRNA in breast cancer tissues was significantly lower than that in adjacent normal tissues (P <0.001). With the occurrence of lymph node metastasis, the expression of ING4 mRNA gradually decreased, with significant difference between the groups (P <0.01). ING4 mRNA expression decreased with the clinical stage of breast cancer increased. Conclusion ING4 gene can be used as an important biological indicator of breast cancer, contribute to the understanding of the biological behavior of breast cancer, and thus guide the clinical treatment.