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目的比较在体和离体2种测量方法测定氯丙烯(AC)亚慢性中毒大鼠坐骨神经的电生理指标的改变。方法90只Wistar雄性大鼠随机分为对照组(n=50)和染毒组(n=40),染毒组大鼠以200mg/kg剂量AC灌胃,每周3次,在染毒3、6、9、12周,分别采用在体和离体2种方法测定相应对照组和染毒组大鼠坐骨神经传导速度(NCV)、复合动作电位峰值(CAPA)、潜伏期(PL)、时程(TC)、阈电位(TP)及最大刺激电位(MSP)等指标。结果与对照组相比,在染毒6、9和12周,NCV在体测定分别降低23.6%、40.4%和48.6%,离体测定染毒12周,NCV降低15.4%,差异均有统计学意义(P<0.05)。染毒前9周,在体测定CAPA无明显变化,染毒第12周,与对照组相比下降了31.7%,染毒6、9、12周,离体测定CAPA与对照组比较分别降低31.7%、38.9%和58.9%,差异均有统计学意义(P<0.05,P<0.01)。PL在体测定第9、12周增加22.6%和40.7%,离体测定第12周增加8.0%,差异均有统计学意义(P<0.01,P<0.05);TC在体测定第6、9、12周分别增加22.5%、34.6%和47.5%,离体测定第12周增加19.5%,差异均有统计学意义(P<0.01);第9、12周,TP在体测定分别增加32.3%和40.0%,离体测定分别增加29.2%和35.6%,差异有统计学意义(P<0.05);MSP在体测定分别增加69.0%和86.5%,离体测定分别增加52.0%和61.9%,差异均有统计学意义(P<0.01)。结论2种测定方法均表明AC可明显改变大鼠坐骨神经电生理特性,并且具有明显的时间依赖性,但其测定的敏感指标不同。在体测定NCV是最敏感指标;离体测定CAPA是最敏感指标,可为AC中毒性神经病早期诊断提供依据。
Objective To compare the change of electrophysiological indexes of sciatic nerve in rats with chronic poisoning of allyl chloride (AC) by in vitro and in vivo measurement methods. Methods Ninety Wistar male rats were randomly divided into control group (n = 50) and exposure group (n = 40). The rats in the exposure group were administered intragastrically with 200mg / kg AC 3 times a week, (NCV), peak of compound action potential (CAPA), latency (PL), duration of injury, and time course of the rats in the corresponding control and exposure groups were measured by in vitro and in vivo methods. (TC), threshold potential (TP) and maximum stimulation potential (MSP) and other indicators. Results Compared with the control group, the in vivo measurement of NCV decreased by 23.6%, 40.4% and 48.6% at 6, 9 and 12 weeks of exposure, respectively, while the NCV decreased by 15.4% in vitro Significance (P <0.05). At 9 weeks before exposure, there was no significant change in CAPA in vivo. At the 12th week of exposure, the percentage of CAPA was decreased by 31.7% compared with the control group and was 6, 9 and 12 weeks after exposure, respectively. Compared with the control group, CAPA decreased by 31.7 %, 38.9% and 58.9%, respectively, with statistical significance (P <0.05, P <0.01). PL increased by 22.6% and 40.7% at the 9th and 12th week, respectively, and increased 8.0% at the 12th week in vitro (P <0.01, P <0.05) , Respectively, increased by 22.5%, 34.6% and 47.5% at 12 weeks, and increased by 19.5% at the 12th week in vitro (P <0.01). At the 9th and 12th week, TP increased by 32.3% And 40.0%, respectively. The in vitro measurements were increased by 29.2% and 35.6%, respectively, with significant difference (P <0.05). The in vivo measurements of MSP increased by 69.0% and 86.5%, respectively, and the in vitro measurements increased by 52.0% and 61.9% All were statistically significant (P <0.01). Conclusion Both methods indicate that AC can significantly change the electrophysiological characteristics of the sciatic nerve in rats, and has a clear time-dependent manner, but the sensitivity of the determination of different indicators. In vivo determination of NCV is the most sensitive index; In vitro determination of CAPA is the most sensitive index, which can provide evidence for the early diagnosis of AC-toxic neuropathy.