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目的建立幽门螺杆菌(H.pylori)慢性感染胃上皮细胞模型,并分析H.pylori慢性感染与胃上皮细胞凋亡的关系.方法首先用H.pylori SSI与人非肿瘤性胃上皮细胞株GES-1共培养16周,建立GES-1模型细胞,然后采用流式细胞术分析这种模型细胞对H.pylori和其它肠道细菌以及抗肿瘤药物凋亡诱导剂的应答.结果成功获得了H.pylori慢性感染胃上皮细胞模型(GES-1模型细胞).这种模型细胞不仅对H.pylori诱导的凋亡耐受,而且对其他肠道细菌和一些凋亡诱导剂也是耐受的。结论幽门螺杆菌慢性感染诱导胃上皮细胞产生凋亡耐受,由此可能增加了胃上皮细胞癌变的危险性.
Objective To establish a chronic gastric epithelial cell model of H. pylori infection and to analyze the relationship between chronic infection of H.pylori and gastric epithelial cell apoptosis.Methods The expression of H.pylori in gastric epithelial cells -1 were co-cultured for 16 weeks to establish GES-1 model cells, and then analyzed by flow cytometry of H.pylori and other intestinal bacteria and anti-tumor drugs apoptosis inducer response.Results H Chronic infection of the gastric epithelial cell model (GES-1 model cells) with pylori This model cell is not only resistant to H. pylori-induced apoptosis but also to other gut bacteria and some apoptosis-inducing agents. Conclusion Chronic Helicobacter pylori infection induced apoptosis in gastric epithelial cells, which may increase the risk of carcinogenesis of gastric epithelial cells.