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目的研究显齿蛇葡萄总黄酮(TFAG)降血糖的效果及其抗氧化能力。方法体外测定利用超声波辅助提取并经D-101大孔树脂纯化得到的TFAG对α-葡萄糖苷酶和α-淀粉酶活性的影响;体内降血糖实验采用链脲佐菌素(STZ)诱导小鼠糖尿病模型,TFAG不同剂量(100、200、400 mg·kg~(-1))连续给药15 d,分别于灌胃0、3、6、9、12、15 d时尾部动脉取血测定血糖,在给药后16 d时,以眼眶采血测定血清胰岛素含量及各项生化指标,并取胰腺组织做HE染色观察,另外取肝脏测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)的含量。结果 TFAG能抑制α-葡萄糖苷酶和α-淀粉酶的活性,且TFAG对α-葡萄糖苷酶的抑制作用较对α-淀粉酶的抑制作用要强。体内实验表明,TFAG能显著降低糖尿病小鼠的血糖、低密度脂蛋白(LDL)、总胆固醇(TC)、甘油三酯(TG)的浓度,增加胰岛素、高密度脂蛋白(HDL)的浓度;HE染色结果显示,TFAG对糖尿病小鼠胰岛细胞具有一定的修复作用;TFAG能使肝组织中MDA的含量明显下降,而SOD、CAT的活性显著升高。结论 TFAG具有显著的降血糖作用和抗氧化能力,能修复胰岛细胞,是一种极具开发价值的药食兼用原材料。
Objective To study the hypoglycemic effect and anti-oxidative ability of TFAG. Methods The effect of TFAG purified by D-101 macroporous resin on α-glucosidase and α-amylase activity was tested in vitro. In vivo hypoglycemic experiments were induced by streptozotocin (STZ) Diabetic model and different dosage of TFAG (100,200,400 mg · kg -1) for 15 consecutive days. Blood was taken from the tail artery at 0, 3, 6, 9, 12 and 15 d after gavage, respectively At 16 d after administration, serum insulin levels and various biochemical indexes were determined by orbital blood sampling. Pancreatic tissues were harvested for HE staining. The activities of superoxide dismutase (SOD), catalase (CAT) ) And malondialdehyde (MDA) content. Results TFAG inhibited the activity of α-glucosidase and α-amylase, and the inhibitory effect of TFAG on α-glucosidase was stronger than that of α-amylase. In vivo experiments showed that TFAG could significantly reduce the concentration of blood glucose, LDL, TC and TG in diabetic mice and increase the concentrations of insulin and HDL. HE staining showed that TFAG could repair pancreatic islet cells in diabetic mice; TFAG could significantly decrease the content of MDA and the activity of SOD and CAT in liver tissue. Conclusion TFAG has a significant hypoglycemic effect and antioxidant capacity, can repair islet cells, is a very valuable combination of both raw and processed food and medicine.