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采用结晶紫染色检测结核分枝杆菌生物被膜的形成能力,实时荧光定量PCR(qRT-PCR)检测生物被膜态的不同结核分枝杆菌Rv3519表达水平,分析Rv3519基因表达与结核分枝杆菌生物被膜形成能力的关系。结果表明:临床分离株产膜能力较H37Rv明显偏低,差异显著(P<0.01);临床分离株之间生物被膜产量差异不显著(P>0.05);生物被膜态的分离株较标准株Rv3519基因表达量偏低,差异显著(P<0.01)。说明结核分枝杆菌生物被膜形成能力与Rv3519基因表达水平相关,Rv3519基因可以作为结核分枝杆菌生物被膜形成能力的潜在分子诊断标志。
The formation ability of Mycobacterium tuberculosis biofilm was detected by crystal violet staining. The expression level of Mycobacterium tuberculosis Rv3519 in biofilm was detected by real-time quantitative PCR (qRT-PCR). The expression of Rv3519 gene and the formation of Mycobacterium tuberculosis biofilm were analyzed Ability of the relationship. The results showed that the membrane-forming ability of clinical isolates was significantly lower than that of H37Rv (P <0.01), and the biofilm yield was not significantly different between clinical isolates (P> 0.05) Gene expression was low, the difference was significant (P <0.01). These results suggest that the ability of Mycobacterium tuberculosis biofilm formation is related to the expression level of Rv3519, and Rv3519 can be used as a potential molecular diagnostic marker for the biofilm formation ability of Mycobacterium tuberculosis.