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目的:探讨大鼠离体肾灌流体系中尿肝型脂肪酸结合蛋白与马兜铃酸(AA)肾损伤的关系。方法:建立大鼠离体肾灌流模型,按5 mL.min-1恒速灌流,空白组给予正常灌流液,马兜铃酸组给予7 mg.(50 mL)-1,灌流给药10 min后,不同时间点收集灌流液和尿液,分别检测钾、钠、氯离子浓度,计算离子清除率,ELISA方法检测尿液肝型脂肪酸结合蛋白浓度。结果:马兜铃酸组钾、钠离子清除率在给药后0.5h明显下降,分别从给药前的(99.54±14.55)%和(27.93±6.66)%下降至(33.16±9.59)%和(9.70±2.62)%,与空白组相比差异显著;尿肝型脂肪酸结合蛋白在给药灌流后即刻明显升高,从给药前(7.42±0.12)ng.L-1升高至(8.18±0.47)ng.L-1,与空白组相比差异显著。结论:尿肝型脂肪酸结合蛋白在评价马兜铃酸致急性肾小管损伤方面优于传统指标离子清除率,是一个早期且敏感的肾小管损伤生物标志物。
Objective: To investigate the relationship between urinary hepatic fatty acid binding protein and aristolochic acid (AA) injury in isolated rat renal perfusion system. Methods: Rat isolated perfused renal perfusion model was established. Perfusion was performed at a constant speed of 5 mL.min-1. Normal perfusate was given to the blank control group. The rats in the aristolochic group received 7 mg. (50 mL) The perfusate and urine were collected at different time points. The concentrations of potassium, sodium and chloride were detected and the ion clearance was calculated. ELISA was used to detect the concentration of fatty acid-binding protein in urine. Results: The clearance rate of potassium and sodium in aristolochic acid group decreased obviously at 0.5h after administration, and decreased from (99.54 ± 14.55)% and (27.93 ± 6.66)% to (33.16 ± 9.59)% and (9.70 ± 2.62)%, which was significantly different from that of the blank group. Urinary hepatic fatty acid-binding protein increased significantly immediately after administration (7.42 ± 0.12) ng.L-1 to (8.18 ± 0.47) ng.L-1, significant difference compared with the blank group. CONCLUSION: Urinary hepatic fatty acid-binding protein is an early and sensitive biomarker of renal tubular injury in evaluating acute renal tubular injury induced by aristolochic acid.