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目的:检测系统性红斑狼疮(SLE)患者外周血中树突状细胞亚型绝对数,分析其与疾病活动程度的相关性。方法:荧光染色标记DC,流式细胞仪检测分析DC亚型绝对数,并分析与SLEDAI、dsDNA、C3、C4的相关性。结果:SLE患者外周血MDC(13630.50±2548.55)和PDC(9196.29±1837.75)较正常对照组(15859.18±2550.55和11362.5±2100.14)明显降低(P<0.05)。静止期MDC和PDC(14503.06±2165.74和9564.75±1748.62)较对照组无明显下降,活动期组两DC亚型(12466.33±2600.32和8514.67±1918.73)较对照组下降明显(P<0.01)。SLE患者外周血MDC和PDC与SLEDAI、dsDNA明显负相关(P<0.01),与C3、C4呈正相关(P<0.05)。结论:DC参与疾病的发生,其绝对数可反映SLE疾病活动程度。
Objective: To detect the absolute number of dendritic cell subsets in peripheral blood of patients with systemic lupus erythematosus (SLE) and to analyze its correlation with disease activity. Methods: DCs were stained with fluorescent staining and analyzed by flow cytometry. The absolute numbers of DC subtypes were analyzed, and their correlations with SLEDAI, dsDNA, C3 and C4 were analyzed. Results: The levels of MDC (13630.50 ± 2548.55) and PDC (9196.29 ± 1837.75) in SLE patients were significantly lower than those in normal controls (15859.18 ± 2550.55 and 11362.5 ± 2100.14) (P <0.05). The quiescent MDC and PDC (14503.06 ± 2165.74 and 9564.75 ± 1748.62) had no significant decrease compared with the control group. The DC subtypes (12466.33 ± 2600.32 and 8514.67 ± 1918.73) in the active group decreased significantly compared with the control group (P <0.01). There was a significant negative correlation between MDC, PDC and SLEDAI and dsDNA in SLE patients (P <0.01), and positively correlated with C3 and C4 (P <0.05). Conclusion: DC is involved in the occurrence of diseases, the absolute number of which reflects the degree of SLE disease activity.