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目的阐述布洛芬在人非小细胞肺癌(non-small cell lung caner,NSCLC)细胞株NCI-H460和乳腺癌细胞株SKBR3中的抗增殖和抑制迁移作用,并对其机制进行探讨。方法运用MTT法检测布洛芬作用下两种不同肿瘤细胞株的细胞增殖抑制情况;运用Transwell方法检测布洛芬作用下两种不同肿瘤细胞株的迁移抑制情况;运用real-time聚合酶链反应(polymerase chain reaction,PCR)和蛋白质印迹方法分析肿瘤细胞survivin和E-cadherin的mRNA和蛋白表达情况。结果 MTT法检测结果显示,布洛芬降低了两种肿瘤细胞株的增殖,并且随着布洛芬药物浓度增高抑制增殖作用增强;Transwell显示,2mmol/L布洛芬作用于两种肿瘤细胞株12h,能够抑制肿瘤细胞株的转移;RT-PCR和蛋白质印迹分析显示,布洛芬能够导致肿瘤细胞survivin的表达降低,使E-cadherin表达增高。结论布洛芬体外能够显著抑制人非小细胞肺癌细胞株NCI-H460和乳腺癌细胞株SKBR3增殖和迁移,这一抑制作用与survivin表达降低和E-cadherin表达增高有关。
Objective To investigate the anti-proliferation and anti-migration effects of ibuprofen in human non-small cell lung caner (NSCLC) cell line NCI-H460 and breast cancer cell line SKBR3, and to explore its mechanism. Methods The inhibitory effect of ibuprofen on the proliferation of two different tumor cell lines was detected by MTT assay. The migration inhibition of two different tumor cell lines was detected by Transwell assay. The real-time polymerase chain reaction The mRNA and protein expressions of survivin and E-cadherin in tumor cells were analyzed by polymerase chain reaction (PCR) and Western blotting. Results The results of MTT assay showed that ibuprofen decreased the proliferation of both tumor cell lines and inhibited the proliferation with the increase of ibuprofen concentration. Transwell showed that 2 mmol / L ibuprofen exerted its effects on two tumor cell lines 12h, could inhibit the metastasis of tumor cell lines. RT-PCR and Western blot analysis showed that ibuprofen could reduce the expression of survivin and increase the expression of E-cadherin in tumor cells. Conclusion Ibuprofen can significantly inhibit the proliferation and migration of human non-small cell lung cancer cell line NCI-H460 and breast cancer cell line SKBR3 in vitro. This inhibitory effect is related to the decrease of survivin expression and the increase of E-cadherin expression.