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目的探讨肺炎合并脓毒症患儿血清降钙素原(PCT)与CD4+CD25+调节性T淋巴细胞(Treg细胞)水平相关性。方法选择诊断为肺炎伴脓毒症患儿80例,依据患儿病情严重程度分为脓毒症组55例与严重脓毒症组25例。入院24h,采用流式细胞仪检测外周血Treg细胞比例,采用免疫化学发光法检测血清PCT水平,比较不同分组患儿Treg细胞与血清PCT水平差异,并分析Treg细胞与血清PCT水平间相关性。结果严重脓毒症组患儿血Treg细胞比例为(57.36±7.03)%高于脓毒症组患儿的(40.52±6.39)%,严重脓毒症组患儿血清PCT为(18.59±3.58)ng/ml高于脓毒症组的(5.26±0.92)ng/ml,差异均有统计学意义(P<0.05)。入院24h患儿血Treg细胞比例与血清PCT水平间呈正相关(r=0.528,P<0.05)。结论肺炎合并脓毒症患儿血清PCT水平与Treg细胞比例存在较好相关性,二者水平高低可以反映患儿病情严重程度。
Objective To investigate the relationship between serum procalcitonin (PCT) and CD4 + CD25 + regulatory T lymphocytes (Treg cells) in children with pneumonia complicated with sepsis. Methods Eighty children diagnosed as pneumonia and sepsis were selected. According to the severity of the illness, they were divided into sepsis group (55 cases) and severe sepsis group (25 cases). At 24 hours after admission, the proportion of Treg cells in peripheral blood was detected by flow cytometry. The levels of PCT in serum were detected by immunochemical chemiluminescence. The differences of PCT levels between Treg cells and serum were compared between different groups. The correlation between Treg cells and serum PCT levels was also analyzed. Results The percentage of Treg cells in severe sepsis group was (57.36 ± 7.03)% higher than that in sepsis group (40.52 ± 6.39%). Serum PCT in severe sepsis group was (18.59 ± 3.58) (5.26 ± 0.92) ng / ml higher than that in sepsis group (P <0.05). There was a positive correlation between the proportion of Treg cells in blood and serum PCT level at admission (r = 0.528, P <0.05). Conclusions There is a good correlation between serum PCT level and the proportion of Treg cells in children with pneumonia complicated with sepsis. The level of PCT can reflect the severity of the illness in children.