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目的探讨转酮醇酶样基因1(TKTL1)在胃癌组织中的表达情况及其与胃癌临床病理特征的关系。方法选取十堰市中西医结合医院2010—2015年收治的胃癌手术患者癌组织标本100例,并将其制作成石蜡标本,分别取癌旁正常组织(经病理证实无癌组织)与癌组织;另取正常胃黏膜组织20例。采用SP免疫组化法测定比较不同组织TKTL1表达,同时分析TKTL1阳性表达与胃癌临床病理特征的关系。结果癌组织TKTL1阳性表达率高于癌旁组织及正常胃黏膜组织(P<0.05);癌旁组织TKTL1阳性表达率高于正常胃黏膜组织(P<0.05)。胃癌患者不同年龄、性别、肿瘤大小、肿瘤分化程度间TKTL1蛋白阳性表达率比较,差异无统计学意义(P>0.05);不同浸润层次、TNM分期与淋巴结转移TKTL1蛋白阳性表达率比较,差异有统计学意义(P<0.05)。结论在胃癌组织中,TKTL1呈高表达,且与浸润层次、TNM分期、淋巴结转移密切相关。
Objective To investigate the expression of transketolase-like gene 1 (TKTL1) in gastric carcinoma and its relationship with clinicopathological features. Methods 100 cases of gastric cancer patients who underwent surgery from 2010 to 2015 in Shiyan Hospital of Integrated Traditional and Western Medicine were selected and made into paraffin-embedded specimens, which were taken from normal tissues adjacent to the tumor (pathologically confirmed non-cancerous tissues) and cancer tissues respectively. Take normal gastric mucosa in 20 cases. The expression of TKTL1 in different tissues was compared by SP immunohistochemistry, and the relationship between the expression of TKTL1 and clinicopathological features of gastric cancer was also analyzed. Results The positive rate of TKTL1 in cancerous tissue was higher than that in paracancerous tissue and normal gastric mucosa (P <0.05). The positive rate of TKTL1 in paracancerous tissue was higher than that in normal gastric mucosa (P <0.05). There was no significant difference in the expression of TKTL1 between different stages of invasion, gender, tumor size and tumor differentiation (P> 0.05). There was significant difference in the expression of TKTL1 between TNM stage and lymph node metastasis Statistical significance (P <0.05). Conclusion The expression of TKTL1 is highly expressed in gastric cancer tissues, and is closely related to the level of invasion, TNM stage and lymph node metastasis.