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分析２２例胃癌癌细胞ＰＣＮＡ表达和ＤＮＡ含量变化与患者预后的关系。使用常规石蜡切片，以单抗Ｐｃ１０。检测ＰＣＮＡ表达，用图像分析系统测定ＤＮＡ含量，将患者按术后生存分为１年组５例，５年组６例，１０年组１１例进行统计。结果：ＰＣＮＡ表达阳性率在１，５，１０年组间皆有明显差异（Ｐ＜０．０１）。ＤＮＡ含量，２Ｃ与≥３ｃ比较在１与５和１０年组间Ｐ＜０．０１，５与１０年组间Ｐ＞０．０５，２ｃ与≥５ｃ比较在１，５，１０年组间皆Ｐ＜０．０１。ＤＮＡ含量变化与ＰＣＮＡ表达里线性密切关系。作者认为，ＤＮＡ含量测定辅以ＰＣＮＡ检测对帮助判断患者的预后更有价值。
The relationship between the expression of PCNA and changes of DNA content in 22 cases of gastric cancer and the prognosis of the patients was analyzed. Conventional paraffin sections were used as the monoclonal antibody Pc10. The expression of PCNA was detected and DNA content was determined by image analysis system. Five patients were divided into 1-year group according to postoperative survival, 6 cases in 5-year group and 11 cases in 10-year group. Results: The positive rate of PCNA expression was significantly different between the 1, 5, and 10 years groups (P<0.01). DNA content, 2C vs. ≥3c, P<0.01 between 1 and 5 and 10 years, P>0.05 between 5 and 10 years, 2c vs. ≥5c, 1,5, and 10 years P<0.01. Changes in DNA content are closely related to PCNA expression. The authors believe that the determination of DNA content supplemented by PCNA testing is more valuable in helping to judge the patient’s prognosis.