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目的 :检测复发性鼻息肉组织中T淋巴细胞亚群的表达 ,探讨T淋巴细胞介导的免疫反应在鼻息肉复发中所起的作用。方法 :应用荧光免疫流式细胞术检测 17例复发性鼻息肉患者鼻息肉组织、外周血T淋巴细胞亚群CD4 + 、CD8+ 、CD4 5RO+ 的表达 ,并与正常人下鼻甲黏膜及外周血的相应指标进行比较。 结果 :复发性鼻息肉组织中有大量T淋巴细胞浸润 [(39.6 5±2 .0 8) % ],而在健康下鼻甲黏膜中几乎未见CD3+ 细胞。在复发性鼻息肉中 ,CD3+ CD4 + 细胞[(6 4.4 46± 5 .2 97) % ]多于CD3+ CD8+ 细胞 [(35 .5 5 4± 5 .2 97) % ](P <0 .0 5 ) ,CD3+ CD4 5RO+ 细胞 [(2 2 .6 49± 2 .789) % ]也显著多于正常人外周血CD3+ CD4 5RO+ 细胞 [(3.896± 0 .384 ) % ](P<0 .0 5 ) ,CD3+ CD4 + /CD3+ CD8+ 比值为 1.95 6± 0 .0 93,复发性鼻息肉患者外周血比值为 2 .36 7±0 .12 8,正常人外周血比值为 1.6 0 6± 0 .0 96 ,其差异均有统计学意义 (均P <0 .0 5 )。结论 :复发性鼻息肉组织中有大量T淋巴细胞表达 ,且T细胞亚群比例失调 ,显示细胞免疫功能的紊乱在鼻息肉的形成与复发中起重要作用
Objective: To detect the expression of T lymphocyte subsets in recurrent nasal polyps and to explore the role of T lymphocyte-mediated immune responses in the recurrence of nasal polyps. Methods: The expression of CD4 +, CD8 + and CD4 + RO + in T lymphocyte subsets of nasal polyps and peripheral blood in 17 patients with recurrent nasal polyps was detected by fluorescence immunofluorescence cytometry. The results were compared with those of normal human inferior turbinate mucosa and peripheral blood The indicators are compared. Results: There was a large amount of T lymphocyte infiltration in recurrent nasal polyps [(39.6 ± 2.58)%], while there was almost no CD3 + cells in healthy inferior turbinate mucosa. In recurrent nasal polyps, the percentage of CD3 + CD4 + cells was significantly higher than that of CD3 + CD8 + cells [(35.54 ± 5.297)%] (P < 5), CD3 + CD4 + 5RO + cells [(2.269 ± 2.789)%] was also significantly higher than that of normal human peripheral blood CD3 + CD4 + 5RO + cells [(3.896 ± 0.384)%] ), The ratio of CD3 + CD4 + / CD3 + CD8 + was 1.95 6 ± 0.93, the ratio of peripheral blood in patients with recurrent nasal polyps was 2.376 ± 0.128, and the ratio of peripheral blood in normal subjects was 1.6 0 6 ± 0 .0 96, the differences were statistically significant (P <0.05). CONCLUSIONS: There is a large number of T lymphocytes in recurrent nasal polyps and the imbalance of T cell subsets, which shows that the disorder of cellular immune function plays an important role in the formation and recurrence of nasal polyps