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目的观察大豆异黄酮对~(60)Co-γ射线照射后小鼠生长和外周血象的影响。方法按体重将80只雌性昆明小鼠随机均分为5组,即正常对照、阳性对照及低、中、高剂量大豆异黄酮实验组。辐照前正常对照、阳性对照组及实验组每天分别以溶剂0.5%羧甲基纤维素钠(CMC-Na)和不同剂量大豆异黄酮连续灌胃14 d,灌胃至第7天,除正常对照组外,其余各组小鼠均接受4.56Gy~(60)Co-γ全身性照射1次,照射后继续灌胃2 d及7天后将动物处死,迅速取血,EDTA抗凝进行检测。结果实验结束时,中、高剂量大豆异黄酮组小鼠体重增重与正常对照无显著差别,但与单纯辐照组相比差别显著(P<0.05);辐照后第7天,大豆异黄酮各实验组白细胞上升均高于单纯辐照组,中剂量实验组显著高于单纯辐照组(P<0.05);仅中剂量大豆异黄酮组网织红细胞照后第7天明显上升,与第2天有明显差异(P<0.01);血红蛋白、血小板及红细胞虽各组间无明显差异,但低、中剂量大豆异黄酮组血红蛋白与正常对照组相近;中剂量组血小板下降幅度在各受辐照组中最小;单纯辐照组红细胞低于各大豆异黄酮干预组,中剂量干预组最接近正常对照组。结论大豆异黄酮对受辐照小鼠的血液系统有一定的保护效果,以中剂量组效果较好,且可预防辐射对生长发育的不良影响。
Objective To observe the effect of soybean isoflavones on the growth and peripheral blood of mice irradiated by 60Co-γ rays. Methods Eighty female Kunming mice were randomly divided into five groups according to body weight: normal control, positive control and low, medium and high dose soy isoflavones experimental group. Before irradiation, the normal control, positive control group and experimental group were treated with 0.5% carboxymethylcellulose sodium (CMC-Na) and different doses of soy isoflavones for 14 days, respectively, and the rats were intragastrically administrated to the 7th day. Outside the control group, mice in the other groups received systemic irradiation of 4.56 Gy 60Co-γ once. After irradiation, the animals were continued for 2 days and 7 days after the irradiation, and the animals were sacrificed. Blood was quickly taken and EDTA was used for anticoagulation. Results At the end of the experiment, there was no significant difference in body weight gain between the middle and high dose soy isoflavone groups and the normal controls, but there was a significant difference compared with the irradiation alone group (P<0.05). On the 7th day after irradiation, the soybean was different. The leukocyte rises in the experimental groups were higher than those in the irradiation alone group, and the middle dose group was significantly higher than that in the irradiation alone group (P<0.05). Only the middle dose soy isoflavone group was significantly increased on the 7th day after reticulocyte irradiation. There was a significant difference on the second day (P<0.01). Although hemoglobin, platelets, and red blood cells did not differ significantly among the groups, the low and middle doses of soy isoflavones had similar levels of hemoglobin to the normal control group; the median dose group had a decrease in platelet count in each group. In the irradiation group, the red blood cells were lower than those in the soybean isoflavone intervention group, and the middle dose group was the closest to the normal control group. Conclusion Soybean isoflavone has a protective effect on the blood system of irradiated mice. The effect of the middle dose group is better, and it can prevent the adverse effects of radiation on growth and development.