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红根草是一个有重要药用价值的珍稀濒危药材植物。为了更好地了解红根草野生和组培快繁种质的遗传多样性信息,本文用RAPD和ISSR分子标记技术,对红根草4个野生种群及一个离体快繁群体进行遗传多样性分析,为物种保护和繁育提供理论依据。结果显示,在物种水平上,该物种的遗传多样性水平中等,Nei基因多样性指数(H)、Shannon信息多样性指数(I)和多态性位点百分率(PPL)分别为0.237/0.248(RAPD/ISSR,下同)、0.365/0.380和78.4%/81.1%;遗传变异大多数(70.5%/81.5%)发生在种群内、少部分(29.5%/18.5%)发生在种群间;野生种群的基因流为1.21/2.20,但UPGMA聚类分析结果表明,距离35km以上的种群遗传分化明显,因此推测基因流动主要存在于种群内,地理距离是种群分化的主要原因。在居群水平上,H、I和PPL三项遗传多样性参数分别为0.167/0.202、0.253/0.303和51.7%/58.0%;离体快繁群体的RAPD分析结果显示,其遗传多样性高于其原野生种群,这一结果暗示,离体快繁过程中可能发生了体细胞变异,这些变异与RAPD-PCR区域有关。
Red grass is an important medicinal value of rare and endangered medicinal plants. In order to better understand the genetic diversity of wild and tissue culture cultivars, we used RAPD and ISSR markers to analyze the genetic diversity of four wild populations and one in vitro propagation population. Analysis, provide a theoretical basis for species protection and breeding. The results showed that the genetic diversity of the species was moderate at the species level, and the Nei gene diversity index (H), Shannon’s information diversity index (I) and the percentage of polymorphic loci (PPL) were 0.237 / 0.248 RAPD / ISSR, the same below), 0.365 / 0.380 and 78.4% / 81.1% respectively. The majority of genetic variation (70.5% / 81.5%) occurred in the population while a small proportion (29.5% / 18.5% The gene flow was 1.21 / 2.20, but UPGMA clustering analysis showed that the genetic differentiation was obvious in the population of more than 35km. Therefore, it was speculated that the gene flow mainly existed in the population, and the geographical distance was the main reason of population differentiation. At population level, the three genetic diversity parameters of H, I and PPL were 0.167 / 0.202,0.253 / 0.303 and 51.7% / 58.0%, respectively. The results of RAPD analysis showed that the genetic diversity was higher The original wild population, the results suggest that in vitro rapid propagation of somatic mutations may occur, these mutations and RAPD-PCR region.