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目的考察玉郎伞多糖(YLSPS)对小鼠产生的毒性反应及死亡分布并探讨YLSPS配伍环磷酰胺后的毒性变化规律。方法按照体重将小鼠随机分为2组:给药组(YLSPS:25 g·kg~(-1))及对照组(30 mL·kg~(-1)),每组20只,用最大耐受量(MTD),进行YLSPS单次灌喂给药毒性实验,2周后观察其死亡率。用均匀设计和固定环磷酰胺LD_(50)剂量联合YLSPS的不同剂量配比两种方法,按照体重将小鼠随机均匀分7组,每组10只,按各组要求以20 mL·kg~(-1)灌喂YLSPS、腹腔注射环磷酰胺,以小鼠死亡率为指标,进行急性毒性评价。结果 YLSPS的最大耐受量为25 g·kg~(-1)·d~(-1),而且无明显毒性反应。随着YLSPS给药剂量的增多,小鼠的死亡率呈下降趋势。固定环磷酰胺LD_(50)与YLSPS不同配比的减毒作用,小鼠死亡率在一定范围内随着YLSPS剂量的增加而降低,尤其在YLSPS-CTX为≥1∶1时,尤其显著。结论 YLSPS低毒安全,配伍后能使环磷酰胺的毒性降低。
Objective To investigate the toxicity and mortality distribution of YLSPS in mice and to explore the changes of toxicity of YLSPS combined with cyclophosphamide. Methods The mice were randomly divided into 2 groups according to their body weight: administration group (YLSPS: 25 g · kg ~ (-1)) and control group (30 mL · kg ~ (-1)), Tolerance (MTD), YLSPS single feeding toxicity test, two weeks after the observation of its mortality. The mice were randomly divided into 7 groups according to body weight, with 10 mice in each group. The mice were randomly divided into two groups according to the dose of 20 mL · kg ~ (-1) YLSPS, intraperitoneal injection of cyclophosphamide, the mortality rate of mice as an indicator for acute toxicity. Results The maximum tolerated dose of YLSPS was 25 g · kg -1 (-1) d -1, with no obvious toxic reaction. With the increase of dose of YLSPS, the mortality rate of mice showed a decreasing trend. The attenuated effects of different doses of cyclophosphamide LD_ (50) and YLSPS in mice decreased in a certain range with the increase of YLSPS dose, especially when YLSPS-CTX was ≥1:1. Conclusion YLSPS is safe and safe, and the compatibility of cyclophosphamide can be reduced after compatibility.