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目的:建立续断通络胶囊质量标准。方法:采用薄层色谱法对处方中的狗脊、续断、白芍、槲寄生进行了定性鉴别;采用HPLC,以川续断皂苷Ⅵ为评价指标,色谱柱Agilent C18(4.6 mm×250 mm,5μm),流动相乙腈-水(30∶70),检测波长212 nm,流速1.0 mL.min-1进行含量测定。结果:供试品色谱中,在与对照品或对照药材色谱相应位置上显相同颜色的斑点,阴性无干扰。川续断皂苷Ⅵ在0.45～3.6μg线性关系良好(r=0.999 9),平均加样回收率为98.31%,RSD 1.42%。结论:方法简便、专属性强、重复性好,可有效的控制续断通络胶囊的质量。
Objective: To establish the quality standards of Duan Tong Tongluo capsule. Methods: TLC, HPLC, Daphniphyllum and Mistletoe were identified qualitatively. HPLC was used to evaluate the effect of Daphniphyllum saponin Ⅵ on the chromatographic column Agilent C18 (4.6 mm × 250 mm , 5μm). The mobile phase was acetonitrile-water (30:70). The detection wavelength was at 212 nm and the flow rate was 1.0 mL.min-1. Results: for the test product chromatography, and the reference substance or reference drug chromatographic position was the same color spots, negative non-interference. The linear relationship of Daphniphyllum saponins Ⅵ in 0.45 ~ 3.6μg was good (r = 0.999 9), the average recovery was 98.31% and the RSD was 1.42%. Conclusion: The method is simple, specific and reproducible, which can effectively control the quality of Duan Tong Tong Luo capsule.