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利用大鼠模型研究糖尿病对尿道功能的损害,以及病程长短对其的影响.雌性SD大鼠(250~275 g),链脲霉素(STZ)65 mg/kg腹腔注射得到糖尿病大鼠模型,5周或10周后在乌拉坦麻醉下利用三导管系统,隔离地测定STZ-糖尿病大鼠和正常对照大鼠膀胱等容收缩状态下的膀胱内压,尿道充盈压和括约肌肌电图(EUS-EMG).静脉注射横纹肌阻断剂-αbungarotoxin(BGT)后再次测定.与正常对照大鼠对比,5周和10周糖尿病大鼠膀胱内压峰值及平滑肌收缩幅度明显降低,并且尿道括约肌的高频振动收缩(HFOs)出现不规则变化.有3只(27%)5周的糖尿病大鼠和3只(33%)10周的糖尿病大鼠出现逼尿肌-括约肌失协调(detrusor-sphincter dyssyn-ergia,DSD).BGT阻断尿道外括约肌后,糖尿病大鼠的尿道充盈压(UPP)有更明显的下降.提示了糖尿病损伤尿道括约肌功能,引起排尿时尿道阻力增加,导致排尿功能的损伤.早期干预有助于防止糖尿病引起的排尿功能的进一步恶化.
Using rat model to study the damage of urethral function and the duration of diabetes mellitus, diabetic rats were induced by intraperitoneal injection of female SD rats (250-275 g) and streptozotocin (STZ) at 65 mg / kg, Five weeks or ten weeks later, urinary bladder pressure, urinary filling pressure and sphincter electromyography (EUS) were measured in isolated contractile states of bladder in STZ-diabetic rats and normal control rats under the urethane anesthesia under urethane anesthesia. -EMG) .After intravenous administration of rhabdomyolinergic blocker-αbungarotoxin (BGT), the peak value of bladder pressure and the amplitude of smooth muscle contraction in diabetic rats at 5 weeks and 10 weeks were significantly lower than those in normal control rats, and the high urethral sphincter There were irregular changes in frequency vibrational constriction (HFOs). Detrusor-sphincter dyssyn was found in 3 (27%) of 5-week diabetic rats and 3 (33%) of 10-week diabetic rats -ergia, DSD) .BGT blocked the urethral sphincter, diabetic rat urethral filling pressure (UPP) have a more significant decrease, suggesting that diabetic urethral sphincter injury, urinary resistance caused by increased urination, resulting in urinary tract injury Early intervention helps prevent it Further deterioration of urinary function caused by diabetes.