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目的:对半固体保存的模拟肛拭子样品进行食源性致病菌的检测,确定引起此次食物中毒的原因,通过实验室间的比对试验,以提高突发公共卫生事件的食源性致病菌的检测能力。方法:根据《食品卫生微生物学检验》GB/T4789.4、7、8、10、30、36-2008、《食品卫生微生物学检验》GB/T4789.5、6、11、14-2003对样品进行增菌分离,观察各平板上的菌落特征,挑取可疑菌落进行生化试验,血清凝集,染色镜检。结果:综合以上得出,本半固体培养基共检出二种菌种:弗氏柠檬酸杆菌;小肠结肠炎耶尔森氏菌。结论:本次食物中毒事件,患者是由于食用了被致病菌污染的食物所引起。
OBJECTIVE: To test the food-borne pathogens of the simulated anal swabs stored in semi-solid and determine the cause of the food poisoning through laboratory comparison test to improve the food source of public health emergencies Sexual bacteria detection ability. Methods: According to “Microbiological examination of food hygiene” GB / T4789.4,7,8,10,30,36-2008, “Microbiological examination of food hygiene” GB / T4789.5,6,11,14-2003 samples For enrichment separation, observe the characteristics of the colonies on each plate, pick suspicious colonies for biochemical tests, serum agglutination, staining microscopy. Results: Based on the above, two kinds of strains were identified in the semi-solid medium: Citrobacter freundii and Yersinia enterocolitica. Conclusion: This food poisoning incident was caused by the consumption of food contaminated by pathogenic bacteria.