论文部分内容阅读
WB29是由农家二棱大麦(Hordeum vulgare ssp.distichon Hsü.)与普通小麦(Triticum aestivum L.)7182回交多代选育的矮秆种质,平均株高57 cm,为明确其矮秆性状遗传规律,结合细胞学鉴定、田间株高调查、赤霉素敏感性鉴定和分子标记技术对该种质进行了鉴定。细胞学鉴定结果表明,WB29根尖染色体数为2n=42,用大麦基因组DNA为探针对WB29根尖细胞染色体进行基因组原位杂交(genomic in situ hybridization,GISH)未发现大麦杂交信号;大麦特异序列标签位点(specific sequence-tagged site,STS)标记ABG459(2HS)在WB29中扩增出了大麦特征条带。利用10对小麦矮秆基因(reduced height gene,Rht)标记对农家二棱大麦、7182、WB29和中国春鉴定,结果7182和WB29检测出含有Rht-D1b,而其他材料不携带任何所检测的基因。通过WB29与中国春杂交获得BC1F1和F2分离群体,对这两个分离群体进行统计分析发现WB29的矮秆性状受1对不完全显性基因控制。赤霉素鉴定结果表明,该矮秆种质为赤霉素不敏感型。对F2群体利用引物ABG459进行筛选后鉴定出322株含有与WB29相同的大麦特征条带;同时对F2中Rht-D1b基因进行了检测,发现343株含有该基因。连锁分析结果表明,ABG459与WB29中主效矮秆基因连锁,遗传距离约为7.0 c M,而Rht-D1b与该主效矮秆基因不连锁。以上结果证实,WB29中携带有大麦2HS染色体遗传物质,WB29中矮秆基因可能位于大麦2HS染色体上或其插入位点附近,且该基因对降低WB29的株高起到了一定作用。本研究明确了WB29为小麦-大麦杂种后代,此材料的育成不仅丰富了小麦-大麦中间材料的种质资源,而且该材料中的主效矮秆基因可能来自于大麦,可能为一新的矮源,对丰富矮秆种质资源和扩大小麦育种中矮秆基因的利用范围具有一定意义。
WB29 is a dwarf germplasm that was selected back from multiple cross generations of Hordeum vulgare ssp. Duichichon Hsü. And Triticum aestivum L. 7182 with an average plant height of 57 cm. To identify its dwarf traits The germplasm was identified by the genetic rule, combined with cytological identification, field plant height survey, gibberellin sensitivity identification and molecular marker technology. Cytological identification results showed that WB29 root chromosome number was 2n = 42, barley hybridization signal was not found by genomic in situ hybridization (GISH) of barley genomic DNA probe with barley genomic DNA as probe. The specific sequence-tagged site (STS) marker ABG459 (2HS) amplified barley bands in WB29. The results showed that Rht-D1b was detected in 7182 and WB29, but none of the other materials did not carry any of the detected genes, using 10 pairs of reduced height gene (Rht) . BC1F1 and F2 segregating populations were obtained by crossing WB29 with Chinese spring, and statistical analysis of the two segregating populations showed that the dwarf trait of WB29 was controlled by one pair of incomplete dominant genes. The result of gibberellin identification showed that the dwarf germplasm was not sensitive to gibberellin. After F2 population was screened by primer ABG459, 322 barley bands containing the same barley as WB29 were identified. At the same time, Rht-D1b gene in F2 was detected and found to contain 343 genes. The results of linkage analysis showed that ABG459 was linked with the main dwarf gene in WB29 with a genetic distance of 7.0 cM, while Rht-D1b was not linked with the main dwarf gene. The above results confirmed that WB29 carried the 2HS chromosome genetic material of barley, and the WB29 dwarf gene may be located on the 2HS chromosome of barley or near its insertion site, and the gene played a role in reducing the plant height of WB29. This study confirmed that WB29 is a wheat-barley hybrid progeny. Breeding of this material not only enriches the germplasm resources of the wheat-barley intermediate material, but also the major-effect dwarf gene in this material may be derived from barley and may be a new short It is of great significance to enrich the dwarf germplasm resources and expand the utilization range of dwarfing genes in wheat breeding.