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目的 测定复方环丙沙星滴鼻液中乳酸环丙沙星、马来酸氯苯那敏、盐酸麻黄碱的含量。方法 高效液相色谱法 ,采用YWG -C18(10 μm ,4 .6mm× 15 0mm)色谱柱 ,以 0 .0 1mol·L-1NaH2 PO4-甲醇 -乙腈 (30 0∶16 0∶4 0 ,三乙胺 0 .5ml,磷酸调pH为 3.0 )为流动相 ,检测波长为 2 5 7nm ,内标法峰高定量。结果 本文可同时测定 3组分的含量。乳酸环丙沙星 0 .0 12～ 0 .0 6mg·ml-1马来酸氯苯那敏 0 .0 3～ 0 .15mg·ml-1,盐酸麻黄碱 0 .1～ 0 .5mg·ml-1范围内 ,峰高比与其浓度呈良好的线性关系 ;平均回收率分别为 10 0 .18%、99.85 %、10 1.6 3% ;RSD分别为 0 .71%、1.2 0 %、1.10 %。结论 方法可靠 ,数据准确 ,灵敏度高 ,重现性好
Objective To determine the contents of ciprofloxacin lactate, chlorpheniramine maleate and ephedrine hydrochloride in compound ciprofloxacin nasal drops. Methods High performance liquid chromatography (HPLC) was performed on a YWG-C18 (10 μm, 4.6 mm × 150 mm) column with 0.1 mol·L-1 NaH2PO4-methanol- Ethylamine 0.5ml, phosphoric acid adjusted to pH 3.0) as the mobile phase, detection wavelength of 257nm, internal standard peak height quantitative. Results This article can determine the content of 3 components. Lactic acid ciprofloxacin 0 .0 12 ~ 0. 6mg · ml-1 chlorpheniramine maleate 0. 0 3 ~ 0 .15mg · ml-1, ephedrine hydrochloride 0.1 ~ 0.5mg · ml -1. The average recoveries were 100.18%, 99.85% and 10 1.6 3%, respectively. The RSDs were 0.71%, 1.2 0% and 1.10%, respectively. Conclusion The method is reliable, the data is accurate, the sensitivity is high and the reproducibility is good