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The properties of the passivation film formed on 316L stainless steel were studied by Electrochemical Impedance Spectroscopy(EIS),Mott-Schottky and Voltammetry measurements in high-temperature acetic acid.The results show that the passivation film formed on 316L stainless steel is stable in 60% acetic acid solution from 25℃to 85℃.As temperature increased.the polarization resistance decreased but the interface capacitance increased.There was hardly any relation between temperature and the intrinsic property semiconductor.The passivation film represents the p-semiconductor properly in the potential interval of-0.5-0.1 V;represents the n-semiconductor property in the potential interval of 0.1-0.9 V;and represents the p-semiconductor property in the potential interval of 0.9-1.1 V.The voltammetry measurements show that the structure of the passivation film is stable when the temperature is lower than 55℃and that its stability decreased when this temperature is exceeded.