像许多转基因高等生物一样,用显微注射方法研制的转基因鱼均为转植基因嵌合体,培育纯合转基因品系已成为研究者们向往和追求的目标。以澎泽鲫(Carassius auratus,Pengze var.)和黄河鲤(Cyprinus carpio,Huanghe var.)为实验材料,以转基因囊胚细胞核或原肠胚细胞核为供体,进行了细胞核移植.检测了转植基因在所获得的移核胚胎和5尾澎泽鲫移核鱼及1尾黄河鲤移核鱼中的存在状况,分析了用转基因早期胚胎细胞核移植方法研制纯合转基因鱼的可能性。 Like many genetically modified organisms, transgenic fish developed by microinjection are all transgenic chimeras. Cultivating homozygous transgenic lines has become a long-awaited goal of researchers. The nuclear transfer was performed using Carassius auratus (Pengze var.) And Yellow river carp (Cyprinus carpio, Huanghe var.) As the experimental material and the nuclear of the blastocyst or the nucleus of the gastrula as the donor, The possibility of developing transgenic mice by nuclear transfer of transgenic embryos was analyzed in the presence of nuclear transfer embryos and five Phytophthora penguinis and one nuclear yellow carp.