Purpose: The aims of this study were to: (1) provide a comprehensive physiological profile of simulated basketball activity and (2) identify temporal changes in player responses in controlled settings. Methods:State-level male basketball players(n=10)completed 4×10 min simulated quarters of basketball activity using a reliable and valid court-based test.A range of physiological(ratings of perceived exertion,blood lactate concentration([BLa-]),blood glucose concentration([BGlu]),heart rate(HR),and hydration)and physical(performance and fatigue indicators for sprint,circuit,and jump activity)measures were collected across testing. Results: Significantly reduced [BLa-] (6.19 ± 2.30 vs. 4.57 ± 2.33 mmol/L; p=0.016) and [BGlu] (6.91 ± 1.57 vs. 5.25 ± 0.81 mmol/L;p=0.009)were evident in the second half.A mean HR of 180.1 ± 5.7 beats/min(90.8% ± 4.0% HRmax)was observed,with a significant increase in vigorous activity (77%-95% HRmax) (11.31 ± 6.91 vs. 13.50 ± 6.75 min; p=0.024) and moderate decrease in near-maximal activity (>95% HRmax)(7.24 ± 7.45 vs.5.01 ± 7.20 min)in the second half.Small increases in performance times accompanied by a significantly lower circuit decrement(11.67% ± 5.55% vs.7.30% ± 2.16%;p=0.032)were apparent in the second half. Conclusion: These data indicate basketball activity imposes higher physiological demands than previously thought and temporal changes in responses might be due to adapted pacing strategies as well as fatigue-mediated mechanisms.