论文部分内容阅读
目的观察亚慢性吸入染毒乙苯对大鼠肝组织的氧化应激损伤。方法将40只健康3周龄SPF级Sprague-Dawley雄性大鼠随机分为4组,分别为对照(新鲜空气)组和低(433.5 mg/m3)、中(4 335 mg/m3)和高(6 500 mg/m3)剂量乙苯染毒组,每组10只。大鼠以笼养方式放入HOPE-MED 8050A型动式染毒柜内,采用吸入方式进行染毒,每天6 h,每周5 d,连续染毒13周。用Beutler改良法测定肝组织还原型谷胱甘肽(GSH)含量,用TBA比色法测定肝组织丙二醛(MDA)含量,在常规显微镜和超微显微镜下观察肝组织结构变化。结果与对照组相比,各剂量乙苯染毒组大鼠肝组织MDA含量升高,GSH含量均降低,差异有统计学意义(P<0.05)。且随着乙苯染毒剂量的升高,大鼠肝组织MDA含量呈上升趋势,GSH含量呈下降趋势。乙苯染毒大鼠肝组织出现肿胀、坏死、部分组织溶解等病理变化;肝细胞呈现核皱缩、线粒体空泡等凋亡形态学特征。结论亚慢性吸入染毒乙苯可诱导大鼠肝组织氧化损伤和细胞凋亡。
Objective To observe the oxidative stress injury induced by subchronic inhalation of ethylbenzene in rat liver tissue. Methods Forty Sprague-Dawley male Sprague-Dawley rats of 3 weeks old were randomly divided into 4 groups: control (fresh air) group and low (433.5 mg / m3), medium (4 335 mg / m3) 6 500 mg / m3) dose ethylbenzene exposure group, 10 rats in each group. The rats were housed in a HOPE-MED 8050A type dynamic cabinet and were exposed by inhalation for 6 hours a day for 5 days a week for 13 weeks. The content of glutathione (GSH) in hepatic tissue was determined by Beutler’s modified method. The content of malondialdehyde (MDA) in liver tissue was determined by TBA colorimetric assay. The changes of hepatic tissue structure were observed under routine microscope and ultramicroscope. Results Compared with the control group, the content of MDA and the content of GSH in the liver of rats in each dose of ethylbenzene treatment group were significantly decreased (P <0.05). And with the increase of ethylbenzene dose, the content of MDA in rat liver increased and the content of GSH decreased. Ethylbenzene-induced liver tissue swelling, necrosis, some tissue lysis and other pathological changes; liver cells showed nuclear shrinkage, mitochondrial vacuoles and other apoptotic morphological characteristics. Conclusion Subchronic inhalation of ethylbenzene can induce oxidative damage and apoptosis of liver in rats.