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以探讨月季切花不同花器官的乙烯生物合成和对乙烯的感受为目的,以切花月季‘Samantha’为试材,进行了乙烯和1-MCP交叉处理,测定花朵各器官乙烯生成量,Rh-ACS3、Rh-ACO1和Rh-ETR3基因的表达。结果表明,1-MCP前处理或后处理均能有效抑制花瓣、雌蕊和花托中由于外源乙烯造成的内源乙烯生成量增加。在基因表达方面,Rh-ACS3、Rh-ACO1和Rh-ETR3在花瓣和雌蕊中对乙烯处理的诱导表达均可被1-MCP前处理或后处理有效抑制,而在花托中,这种抑制主要针对Rh-ETR3。这些结果说明,1-MCP对乙烯的竞争性抑制作用主要发生在花瓣、雌蕊和花托上,其竞争效果在乙烯处理前或乙烯处理后均有效。
In order to explore the ethylene biosynthesis and the ethylene sensation in different floral organs of rose cut flowers, the intercropping of ethylene with 1-MCP was carried out with cut rose ’Samantha’ , Rh-ACO1 and Rh-ETR3 gene expression. The results showed that 1-MCP pretreatment or post-treatment could effectively inhibit the increase of endogenous ethylene production due to exogenous ethylene in petals, pistils and torus. In gene expression, the induction of ethylene treatment by Rh-ACS3, Rh-ACO1 and Rh-ETR3 in the petals and pistils was both effectively inhibited by 1-MCP pretreatment or post-treatment, whereas in the receptacle, this inhibition was mainly For Rh-ETR3. These results indicate that the competitive inhibition of ethylene by 1-MCP mainly occurs on petals, pistils and receptacle, and the competition effect is both before ethylene treatment and after ethylene treatment.