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Phenol is a common industrial chemical produced and transported worldwide largely.Therefore,accidental spillage of phenol in the ocean causes an increasing conc.Microalgae are promising to remove phenol from marine waters.However,temperature and light intensity are two main factors that markedly influence biodegradation in marine environments.In this study,a marine golden alga Isochrysis galbana is selected to research the removal of phenol under different temperatures(10-30°C)and light intensities(0-240 μmol/(m 2 ·s)).The results show that the most suitable temperature and light intensity for phenol removal are 20°C and 180 μmol/(m 2 ·s),respectively,and 100 mg/L of phenol can be completely removed by microalga in 24 h at these conditions.I.galbana can also remove phenol under dark and low-temperature conditions.The removal of phenol by I.galbana at diverse temperatures and light intensities conform to first-order kinetics,and the process under dark conditions conform to zero-order kinetics.Thus,I.galbana can be used in the in-situ bioremediation of polluted seawater by phenol.