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目的:评定香延止痛方治疗原发性痛经的药效学。方法:根据文献建立大、小鼠痛经模型,分为模型组、阿司匹林组(大鼠0.042 g.kg-1,小鼠0.06 g.kg-1),香延止痛方小剂量组(大鼠5.19 g.kg-1,小鼠7.41 g.kg-1)、香延止痛方大剂量组(大鼠10.37 g.kg-1,小鼠14.82 g.kg-1),观察香延止痛方的镇痛作用。用免疫学方法测量大鼠子宫组织中前列腺素E2(PGE2)和前列腺素F2α(PGF2α),观察药物的影响。结果:香延止痛方能显著延长痛经模型小鼠的扭体反应潜伏期,减少扭体反应次数,与模型组比较,差异有显著性(P<0.05)。能显著降低大鼠子宫组织中的PGF2α含量,提高PGE2的含量,降低PGF2α/PGE2,与模型组比较,差异有显著性(P<0.05)。结论:研究显示香延止痛方可能通过提高痛阈及改变子宫中PGE2和PGF2α的含量,起到治疗痛经的作用。
OBJECTIVE: To evaluate the pharmacodynamics of “Shaoyan Zhitong Fang” in the treatment of primary dysmenorrhea. Methods: According to the literature, the model of dysmenorrhea in rats and mice was established and divided into three groups: model group, aspirin group (0.042 g.kg-1, 0.06 g.kg-1) g.kg-1, 7.41 g.kg-1 in mice), high-dose Xiang-yangzhitong prescription group (10.37 g.kg-1 in rats and 14.82 g.kg-1 in mice) Pain effect. Immunoassay was used to measure prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) in rat uterine tissue to observe the effects of drugs. Results: Xiangyan analgesic can significantly prolong the writhing reaction latency and reduce writhing response in mice with dysmenorrhoea. Compared with the model group, the difference was significant (P <0.05). Can significantly reduce the content of PGF2α in uterine tissue, increase the content of PGE2 and decrease the level of PGF2α / PGE2 in rat uterine tissue. There is significant difference between the model group and the control group (P <0.05). Conclusion: The study shows that the Shaoyanzhitong Fang may improve the pain threshold and change the content of PGE2 and PGF2α in the uterus, play a role in the treatment of dysmenorrhea.