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目的:探讨荔枝核提取物成分黄酮类化合物(FLC)体外抑制腺病毒(AdV)增殖的作用。方法:采用细胞病变效应(CPE)、四甲基偶氮唑盐微量酶反应比色法(MTT)等方法检测不同浓度的FLC分别作用于AdV复制周期的3个阶段的抗病毒作用。结果:FLC可在病毒感染后通过抗病毒生物合成方式抑制AdV增殖,其半数细胞毒性浓度(TC50)和半数有效抑制浓度(IC50)分别为116.83mg/L,15.46mg/L,且FLC在1.25-40mg/L浓度范围内随药物浓度增加,其抗病毒作用越明显,病毒抑制率最高达84.31%,治疗指数为7.56。但研究未发现FLC具有抗病毒吸附和直接杀伤病毒的作用。结论:FLC体外可通过抗病毒生物合成的方式明显抑制AdV在人喉癌细胞(HEp-2)中的增殖。FLC有望成为一种新的有效抗腺病毒药物。
Objective: To investigate the inhibitory effect of flavonoids (FLC) on the proliferation of adenovirus (AdV) in vitro. Methods: The antiviral effects of different concentrations of FLC on the three stages of AdV replication cycle were detected by cytopathic effect (CPE) and tetramethylzirconate (MTT) assay. Results: FLC could inhibit the proliferation of AdV by antiviral biosynthesis after virus infection. The half-cell cytotoxicity (TC50) and half effective inhibitory concentration (IC50) of FLC were 116.83 mg / L and 15.46 mg / L, respectively, -40mg / L concentration range with the drug concentration increases, the more obvious antiviral effect, the virus inhibition rate up to 84.31%, the treatment index was 7.56. However, the study did not find FLC has antiviral adsorption and direct anti-virus effect. Conclusion: FLC can inhibit the proliferation of AdV in human laryngeal carcinoma cells (HEp-2) in vitro by antiviral biosynthesis. FLC is expected to become a new and effective anti-adenovirus drug.