病毒类疫苗是将病毒株利用鸡胚、适宜的细胞培养物、动物组织或基因工程细胞培养制备而成。在制备和培养细胞过程中,因细胞培养液中含有丰富的营养成分,容易发生污染,因此,国内外大部分病毒性疫苗在细胞培养阶段通常添加一定浓度的抗生素来抑制细菌、支原体的污染[1]。 Viral vaccine is the use of chicken embryo strains, suitable cell culture, animal tissue or genetically engineered cell culture prepared. In the process of preparing and culturing cells, the cell culture fluid is rich in nutrients and is prone to contamination. Therefore, most of the viral vaccines at home and abroad usually add a certain concentration of antibiotics to inhibit the bacterial and mycoplasma contamination in the cell culture stage [ 1].