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为寻找能无损伤地量化提示抗日本血吸虫疫苗接种后保护作用的测定指标，试从三个方面进行了探索：①通过获取可排除活动性新感染．从而提示免疫保护性存在的高特异、高性能单抗免疫探针，测量宿主体内的循环抗原（CAg）组分，经3农杂交瘤细胞融合、筛选，制备了5株具诊断效能的（IgM）单抗，发现组合应用这些单抗为捕获兼测示抗体，明显优于单个应用．其中IE＋11H＋12G组合显示了在目前多种组合中最佳检测效能．采用Dot－ELISA及新建立的斑点金免疫渗滤法，在样本数（n）分别为184和104的两组健康人群中，分别显示了94．6％及95．2％的高特异性，而在139例及69例两批慢性血吸虫感染者，该两法也分别达到了可以接受的54．7％和60．8％的阳性检出率，也已获得了1株IgG_3同型单抗，A9．初步测试具有较好诊查效果，正在进一步作出鉴定;②为建立采用优质纯化抗原的标准系列，利于定量值测CAg的灵敏度，我们采用Mono－Q高压液相技术纯化分离了SEAj的活性组分，已初步用为测量CAg组分的定量标准参考系列；③通过方法学的改进，借助于BIO－TECH自动酶标仪，建立了测量与抗力相关的特异性IgE及疾病相关的特异性IgG_4的动态ELISA或经标化的终点列读常规，在243例慢性血吸虫病血样测读基础上，经100例健康人测试，?
In order to search for the measurement index that can promptly quantify the protective effect of anti-Schistosoma japonicum vaccine after inoculation, try to explore three aspects: ①New infection can be excluded by activity. The results showed that immunoprotective high-specificity and high-performance monoclonal antibody immuno-probes were used to measure the circulating antigen (CAg) components in the host. Fusion and screening of 3 hybridoma cells resulted in the identification of 5 diagnostic IgM ) MAb and found that the combination of these mAbs was a capture and test antibody, significantly better than a single application. Among them, the combination of IE + 11H + 12G shows the best detection performance in many combinations at present. Using Dot-ELISA and the newly developed Auger Auger method, 94.6% and 95.2% of the high-specificity of the two groups of healthy people with sample numbers (n) of 184 and 104, respectively, In 139 and 69 patients with two groups of chronic schistosomiasis infection, the two methods also reached an acceptable positive rate of 54.7% and 60.8%, respectively, and also obtained an IgG3 isotype monoclonal antibody, A9. The preliminary test has a good diagnostic effect, and further identification is underway. ② To establish a standard series of high quality purified antigens for the quantitative detection of CAg sensitivity, we purified and separated the active components of SEAj using Mono-Q high pressure liquid phase technique , Has been initially used to measure quantitative reference standard series of CAg components; (3) through methodological improvements, with the aid of BIO-TECH automatic microplate reader, established a measure of resistance-specific IgE and disease-specific IgG_4 Dynamic ELISA or the standardization of the end point read routine, in 243 cases of chronic schistosomiasis blood test, based on 100 healthy people to test?