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目的通过研究丙烯醛对DNA分子的损伤,探讨丙烯醛的遗传毒性效应及其分子机制。方法应用单细胞凝胶电泳技术检测丙烯醛引起的DNA断裂、DNA交联以及DNA-蛋白质交联;应用液相色谱-电化学法研究丙烯醛致DNA分子产生氧化损伤标志物8-羟基脱氧鸟苷(8-OHdG)。结果丙烯醛可诱导人外周血淋巴细胞DNA发生链断裂,但不引起DNA-DNA、DNA-蛋白质交联;丙烯醛与小牛胸腺DNA的体外作用较弱,但在铁离子介导下对DNA的氧化能力增强,可产生一定量的8-OHdG加合物;动物实验表明丙烯醛诱导大鼠肺组织DNA氧化损伤生成少量8-OHdG。结论丙烯醛具有直接的遗传毒性效应,产生自由基造成DNA氧化损伤是其遗传毒性效应的主要途径。
Objective To investigate the genotoxicity of acrolein and its molecular mechanism by studying the damage of acrolein to DNA molecules. Methods Single-cell gel electrophoresis was used to detect acrolein-induced DNA fragmentation, DNA cross-linking and DNA-protein cross-linking. The oxidative DNA damage induced by acrolein was detected by liquid chromatography-electrochemistry Glycoside (8-OHdG). Results Acrolein could induce DNA strand breaks in human peripheral blood lymphocytes, but did not cause DNA-DNA and DNA-protein cross-links. Acrolein and calf thymus DNA had weaker in vitro activities, but acted on by iron ions Of the oxidative capacity, can produce a certain amount of 8-OHdG adduct; animal experiments show that acrolein-induced DNA oxidative damage in rat lung tissue to generate a small amount of 8-OHdG. Conclusion Acrolein has a direct genotoxic effect. The main pathway of genotoxic effects of DNA damage caused by free radicals is acrolein.