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目的在实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型中,比较常规T2加权成像(T2-weighted imaging,T2WI)、钆-二乙三胺五醋酸(gadolinium-diethylenetriamine pentaacetic acid,Gd-DTPA)和超顺磁性氧化铁(superparamagnetic iron oxide,SPIO)增强图像之间的差异,探讨巨噬细胞在多发性硬化(multiple sclerosis,MS)炎性活动病灶中的细胞学标志。方法在EAE模型临床症状的亚临床期、初发期、高峰期,13只复发缓解(relapsing-remitting,R-R)EAE大鼠模型组和13只正常对照组大鼠在注入对比剂之前均行常规T2WI扫描,接着分别在其尾静脉注入Gd-DTPA后5min行T1加权成像(T1-weighted imaging,T1WI),再注入SPIO,24h后行T2*WI扫描。扫描完毕后立即处死大鼠取脑,行脑组织切片的ED1免疫组织化学染色和Prussian blue染色。结果 EAE模型组大鼠在第11天出现临床症状(初发期),第14天达到高峰期;MRI检查:SPIO增强图像对EAE病灶的显示较常规T2WI和Gd-DTPA增强图像好。病理学检查:ED1染色,在SPIO显示为低信号的区域内出现了炎症细胞(以巨噬细胞为主)浸润;Prussian blue染色示病灶内巨噬细胞胞质内出现了蓝染颗粒,沉积部位与T2*WI上低信号区对应。对照组大鼠均无异常。结论 SPIO较Gd-DTPA更好地显示EAE模型中炎性活动性病灶内血管周围以巨噬细胞为主的浸润。
Objective To compare the effects of conventional T2-weighted imaging (T2WI) and gadolinium-diethylenetriamine pentaacetic acid (Gd) in experimental autoimmune encephalomyelitis (EAE) -DTPA) and superparamagnetic iron oxide (SPIO) enhanced images to investigate the cytological markers of macrophages in inflammatory lesions of multiple sclerosis (MS). Methods The EAE model of clinical symptoms in subclinical, early onset, peak, 13 relapsing-remitting (EAE) rat model group and 13 normal control rats before injection of contrast agent routine T2WI scan was performed. Then T1-weighted imaging (T1WI) was performed 5 min after Gd-DTPA injection into the caudal vein, then injected into SPIO and T2 * WI scan after 24h. Immediately after the completion of scanning, the rats were sacrificed and their brains were taken out. ED1 immunohistochemistry and Prussian blue staining of brain sections were performed. Results In EAE model group, the clinical symptoms (initial onset) appeared on the eleventh day and peaked on the fourteenth day. The MRI examination showed that the enhanced SPIO images displayed better EAE lesions than the conventional T2WI and Gd-DTPA enhanced images. Pathological examination: ED1 staining showed infiltration of inflammatory cells (predominantly macrophages) in the area where SPIO showed a low signal; Prussian blue staining revealed the presence of blue-stained granules in macrophage cytoplasm within the lesion, Corresponds to the low signal area on T2 * WI. The control group rats were normal. Conclusions Compared with Gd-DTPA, SPIO better displays macrophage-predominant perivascular infiltration in inflammatory active lesions in the EAE model.