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目的:探讨P16ink4a作为肿瘤抑制因子在宫颈液基细胞学(LCT)中的应用。方法:选取120例细胞学检查标本,分别进行液基细胞学检测和P16ink4a免疫细胞化学染色,对阳性标本进行组织学检查。结果:P16ink4a作为分子标记物在宫颈液基细胞学诊断为未见上皮内病变及癌变(NILM)、非典型鳞状上皮细胞(ASC)、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)的阳性表达率分别为0(0/20)、42.5%(17/40)、27.5%(11/40)、95%(19/20),其中ASC、HSIL组的阳性率显著高于LSIL、NILM组(P<0.05)。结论:P16ink4a作为分子标记物在免疫细胞化学染色中提高了对非典型鳞状上皮细胞的识别能力和宫颈癌筛查的准确性。
Objective: To investigate the application of P16ink4a as tumor suppressor in cervical liquid-based cytology (LCT). Methods: 120 cases of cytological specimens were selected for liquid-based cytology and P16ink4a immunocytochemical staining, the positive specimens for histological examination. RESULTS: P16INK4a was diagnosed as cervical intraepithelial neoplasia without cervical intraepithelial neoplasia and carcinogenesis (NILM), atypical squamous cell (ASC), low grade squamous intraepithelial lesion (LSIL), highly squamous The positive rates of HSIL were 0 (0/20), 42.5% (17/40), 27.5% (11/40) and 95% (19/20), respectively The rates were significantly higher than those in LSIL and NILM groups (P <0.05). Conclusion: P16ink4a as a molecular marker in immunocytochemical staining to improve the recognition of atypical squamous cell and cervical cancer screening accuracy.