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目的:检测受体O型蛋白酪氨酸磷酸酶(protein tyrosine phosphatase receptor-type O,PTPRO)在人类肝细胞肝癌(hepatocellular carcinoma,HCC)中的表达水平,研究该基因的表达是否具有性别差异,并是否与HCC肝切除术后肿瘤复发相关,是否可以作为判断预后的指标。方法:①利用实时荧光定量聚合酶链反应和免疫组织化学的方法,分别检测59例男性及22例女性HCC患者肿瘤组织及癌旁组织中PTPRO及雌激素受体(estrogen receptors,ERs)的表达水平;②通过t检验分析PTPRO、ERs在肿瘤、瘤旁及不同性别间的表达差异,并通过相关性分析及F检验了解PTPRO表达与ERs是否具有相关性;③通过Kaplan-Meier曲线研究不同PTPRO表达水平与HCC患者术后复发率之间的关系。结果:①PTPRO、ERα在HCC组织中的mRNA及蛋白水平明显低于癌旁肝硬化组织(P<0.000 1);②HCC癌旁组织中PTPRO、ERα的表达水平,男性显著低于女性(P<0.05);③Kaplan-Meier曲线及Log-rank检验表明HCC癌旁组织中PTPRO水平与HCC复发水平呈负相关(P<0.000 1)。结论:PTPRO是一种具有性别差异性的抑癌基因,可作为判断HCC肝切除术后预后的指标。
OBJECTIVE: To detect the expression of PTPRO in human hepatocellular carcinoma (HCC) and to investigate whether there is a gender difference in the expression of PTPRO (P-tyrosine phosphatase receptor-type O) And whether it is related to tumor recurrence after HCC resection and whether it can be used as an indicator of prognosis. Methods: ①To detect the expression of PTPRO and estrogen receptors (ERs) in 59 cases of male and 22 cases of female HCC by real-time fluorescence quantitative polymerase chain reaction and immunohistochemistry. ②Through t-test, we analyzed the expression of PTPRO and ERs in tumor, tumor and different sexes, and analyzed the relationship between PTPRO expression and ERs by correlation analysis and F test.③Through Kaplan-Meier curve, Relationship between the level and recurrence rate of HCC patients. Results: ① The mRNA and protein levels of PTPRO and ERα in HCC tissues were significantly lower than those in adjacent non-cancerous cirrhotic tissues (P <0.000 1). ② The expressions of PTPRO and ERα in HCC tissues were significantly lower than those in women (P <0.05) ); Kaplan-Meier curve and Log-rank test showed that the level of PTPRO in HCC adjacent tissues was negatively correlated with the recurrence of HCC (P <0.000 1). Conclusion: PTPRO is a kind of sex-specific tumor suppressor gene, which can be used as an index to judge the prognosis of HCC after hepatectomy.