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本实验研究了大鼠肝部分切除后72小时血清氨基酸的代谢变化。实验分三纰:正常对照组、假手术组和肝部分切除组。用氨基酸自动分析仪测定了主动脉、下腔脉静,门静脉和肝静脉血清20种氨基酸浓度。结果表明大鼠肝部分切除后72小时,上述血管血清氨基酸浓度均显著升高。肝摄取氨基酸总量显著增加,门静脉区内脏释放氨基酸总量占肝摄取量的87%。二者呈正相关。外周血管血清必需与非必需氨基酸比值和支链与芳香族氨基酸比值均显著下降。外周组织对支链氨基酸摄取严重抑制,而肝脏摄取则显著增加,这不仅为肝再生提供充分支链氨基酸的供应,而且促进肝蛋白质的合成。门静脉区内脏释放和肝脏摄取丙氨酸的量均显著增加,从而为肝糖原异生提供原料的供应。
This study investigated the changes of serum amino acids 72 h after partial hepatectomy in rats. The experiment was divided into three groups: normal control group, sham operation group and partial hepatectomy group. Amino acid analyzer was used to determine the aorta, inferior vena cava, portal vein and hepatic vein serum concentrations of 20 kinds of amino acids. The results showed that at 72 hours after partial hepatectomy in rats, the above serum concentrations of serum amino acids were significantly increased. Total liver amino acid intake increased significantly, portal vein release of visceral total amino acid accounted for 87% of liver intake. The two are positively correlated. Peripheral vascular serum essential and non-essential amino acids ratio and the ratio of branched-chain aromatic amino acids were significantly decreased. Peripheral tissue severely inhibited the uptake of branched-chain amino acids, while the liver uptake was significantly increased, which not only provided a sufficient supply of branched-chain amino acids for liver regeneration but also promoted hepatic protein synthesis. There was a significant increase in visceral release and hepatic uptake of alanine in the portal vein area, thereby providing a supply of raw material for glycogen in vitro.