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目的:探讨黄芪多糖(APS)对糖尿病大鼠肾脏神经肽Y(NPY)及其Y2受体(Y2R)表达的影响。方法:采用腹腔注射链脲佐菌素(STZ)制备糖尿病大鼠动物模型,随机分为对照组、糖尿病组和黄芪多糖干预组;采用放射免疫法检测血浆和肾皮质NPY水平,实时荧光定量PCR方法检测肾皮质NPYmRNA的表达,免疫组织化学方法检测肾皮质Y2R蛋白表达,同时检测大鼠的血糖、尿白蛋白排泄率(UAER)、肾重/体重。结果:①APS干预能显著削弱糖尿病大鼠血糖、UAER和肾重/体重的增加;②APS干预后显著降低糖尿病大鼠血浆和肾皮质NPY水平(P<0.01);③APS干预组大鼠肾皮质NPYmRNA和Y2R蛋白表达显著低于糖尿病组(P<0.05)。结论:APS对糖尿病肾病的保护作用机制可能与下调肾脏NPY及其Y2R的表达有关。
Objective: To investigate the effect of astragalus polysaccharide (APS) on the expression of neuropeptide Y (NPY) and its receptor Y2R in kidney of diabetic rats. Methods: The animal model of diabetic rats was established by intraperitoneal injection of streptozotocin (STZ) and randomly divided into control group, diabetes mellitus group and astragalus polysaccharide intervention group. Radioimmunoassay was used to detect plasma and renal cortex NPY levels. Real-time quantitative PCR Methods The expression of NPY mRNA in renal cortex was detected. The expression of Y2R protein in renal cortex was detected by immunohistochemical method. The blood glucose, urinary albumin excretion rate (UAER) and renal weight / body weight were also measured. Results: ①APS intervention significantly attenuated the increase of blood glucose, UAER and renal weight / body weight in diabetic rats; ②APS treatment significantly reduced the level of NPY in plasma and renal cortex of diabetic rats (P <0.01); ③APS intervention of renal cortex NPY mRNA and Y2R protein expression was significantly lower than the diabetic group (P <0.05). Conclusion: The protective effect of APS on diabetic nephropathy may be related to the down-regulation of NPY and its expression of Y2R in kidney.