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测定斑蟊素饲喂对菜蛾Plutella xylostella L.幼虫体内主要消化酶和酯酶的影响。结果表明,斑蝥素处理后试虫的主要消化酶类平均活性变化不大,与对照组相比,蛋白酶、脂肪酶平均活性分别被抑制了8.71％和15.69％,α-淀粉酶活性较对照组增加了6.75％,均未达到显著差异水平;酯酶中的磷酸酯酶活性平均降低25.33％,未达显著差异水平,乙酰胆碱酯酶和羧酸酯酶活性显著提高,36h内平均比活力分别提高了74.3％和210.57％,达显著差异水平。试虫中毒后活动能力下降,缓慢趋于死亡,表明斑蟊素具有“软性”杀虫剂的特点。乙酰胆碱酯酶活性的提高原因值得探讨。
To determine the effects of cantharidin feeding on the main digestive enzymes and esterases in Plutella xylostella larvae. The results showed that after the cantharidin treatment, the average activities of major digestive enzymes of the test insects changed little, compared with the control group, the average activity of protease and lipase was inhibited by 8.71% and 15.69%, respectively, and the activity of α-amylase was higher than that of the control group Increased 6.75%, did not reach significant differences; esterase phosphatase activity decreased by an average of 25.33%, did not reach significant differences in levels of acetylcholinesterase and carboxylesterase activity significantly increased within 36h average specific activity increased 74.3% and 210.57% respectively, reaching significant differences. Testis poisoning activity decreased, slow to death, indicating that cantharidin has a “soft” insecticide characteristics. The reason for the increase of acetylcholinesterase activity worth exploring.